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Advanced
Techniques in Structural Biology
This course provides a detailed
presentation of the methodologies of structural biology, including X-ray
crystallography, electron microscopy, NMR spectroscopy, as well as confocal
light microscopy. Laboratory exercises will provide students with hands-on
experience in the techniques of structural biology.4 credit hours - Spring
semester
2 lectures and 1 laboratory
session per week
Suitable for 1st and 2nd
year students
Full
Schedule
Course Director
Xiangpeng Kong
Topics
Basic mathematical tools
Vectors, complex numbers,
3D coordinates, group/symmetry, fourier transformation, radon transformation
Structure determination
by X-ray crystallography
X-ray sources, protein crystals,
space groups, protein crystallization, X-ray data collection, phase problem,
molecular replacement, MIR, MAD, Patterson methods, electron density map,
solvent flattening, model building and refinement
Structure determination
by electron crystallography
Electron microscopy, 2D crystals
and tubes, Cryo-electron microscopy, electron crystallography, single-particle
analysis, electron tomography, atomic models in EM density map
Structure determination
by NMR
Basis for proton spin resonance,
COSY vs. NOESY experiments, spin assignments, structure determination,
solid state and other methods
Structure determination
by confocal microscopy
Immunolabelling of cells,
principles of confocal microscopy, data collection, 3D visuallization
Laboratory exercises
Structure determination of
lysozyme, tomographic reconstruction of a desmosome, confocal through-focus
reconstruction
Instructors
Tim Cardozo
Wenbiao Gan
Stevan Hubbard
Alexej Jerschow
Xiangpeng Kong
Thomas Neubert
David Stokes
Dan Turnbull
Da-Neng Wang
Rui-Ming Xu
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