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Estimating Concentrations of DNA Templates
Using Lambda DNA HindIII digest solution
a Standard:
Total size of Lambda DNA: 48502 bp.
Concentration: 200ng/ul
Load on gel 10ul = 2000ng, 5ul = 1000ng, 2.5ul = 500ng
| Band # |
bp |
% of total size |
Load 2ug=ng of DNA |
Load 1ug=ng of DNA |
Load .5ug=ng of DNA |
| 1 |
23,130 |
47.69% |
954ng |
477ng |
238.5ng |
| 2 |
9,416 |
19.41% |
388ng |
194ng |
97ng |
| 3 |
6,557 |
13.52% |
270ng |
135ng |
67.5ng |
| 4 |
4,361 |
8.99% |
180ng |
90ng |
45ng |
| 5 |
2,322 |
4.79% |
96ng |
48ng |
24ng |
| 6 |
2,027 |
4.18% |
84ng |
42ng |
21ng |
| 7 |
564 |
1.16% |
23ng |
11.5ng |
5.75ng |
| 8 |
125 |
0.26% |
5ng |
2.5ng |
1.25ng |
| Total |
48502 |
100% |
2000ng |
1000ng |
500ng |
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