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Nicolas Manel
Irvington Institute Fellowship Program of the Cancer Research Institute
Former EMBO fellow
PhD University Montpellier II, France
nicolas.manel@med.nyu.edu

I am interested in various aspects of HIV-1 interactions with dendritic cells. Dendritic cells play a fundamental role in driving an appropriate immune response. Interestingly, HIV-1 is very inefficient at infecting dendritic cells. It has been shown that this restriction to HIV-1 can be alleviated by Vpx, a protein absent in HIV-1 but found in the related SIVmac. I am developing genetic approaches to identify the mechanisms by which HIV is restricted in dendritic cells. I am also interested in the innate response to HIV-1. The innate reponses to many pathogens and chemical agents have been identified and are mediated by families of pattern-recognition receptors (PRRs) including TLR, NLR and RLH. However, the innate sensors to HIV-1 remain elusive. I am developing various molecular approaches to identify the innate sensors to HIV-1. Importantly, the ability to manipulate these mechanisms will likely be important to develop better HIV-1 vaccines.

Publications

Ivanov II, Frutos Rde L, Manel N, Yoshinaga K, Rifkin DB, Sartor RB, Finlay BB, Littman DR. Specific microbiota direct the differentiation of IL-17-producing T-helper cells in the mucosa of the small intestine. Cell Host Microbe. 2008 4(4):337-49.

Srivastava S, Swanson SK, Manel N, Florens L, Washburn MP, Skowronski J. Lentiviral Vpx accessory factor targets VprBP/DCAF1 substrate adaptor for cullin 4 E3 ubiquitin ligase to enable macrophage infection. PLoS Pathog. 2008 4(5):e1000059.

Manel N, Unutmaz D, Littman DR. The differentiation of human T(H)-17 cells requires transforming growth factor-beta and induction of the nuclear receptor RORgammat. Nat Immunol. 2008 9(6):641-9. Link to Protocol

Montel-Hagen A, Kinet S, Manel N, Mongellaz C, Prohaska R, Battini JL, Delaunay J, Sitbon M, Taylor N. [Red cell GLUT1 compensates for the lack of vitamin C synthesis in mammals]. Med Sci (Paris). 2008 24(4):434-6.

Montel-Hagen A, Kinet S, Manel N, Mongellaz C, Prohaska R, Battini JL, Delaunay J, Sitbon M, Taylor N. Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. Cell. 2008 132(6):1039-48.

Kinet S, Swainson L, Lavanya M, Mongellaz C, Montel-Hagen A, Craveiro M, Manel N, Battini JL, Sitbon M, Taylor N. Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells. Retrovirology. 2007 4:31.

Boggiano C*, Manel N*, Littman DR. Dendritic cell-mediated trans-enhancement of human immunodeficiency virus type 1 infectivity is independent of DC-SIGN. J Virol. 2007 81(5):2519-23. [*equal contribution]

Manel N, Battini JL, Taylor N, Sitbon M. HTLV-1 tropism and envelope receptor. Oncogene. 24(39):6016-25.

Swainson L, Kinet S, Manel N, Battini JL, Sitbon M, Taylor N. Glucose transporter 1 expression identifies a population of cycling CD4+ CD8+ human thymocytes with high CXCR4-induced chemotaxis. Proc Natl Acad Sci U S A. 2005 102(36):12867-72.

Manel N, Battini JL, Sitbon M. Human T cell leukemia virus envelope binding and virus entry are mediated by distinct domains of the glucose transporter GLUT1. J Biol Chem. 2005 280(32):29025-9.

Kim FJ, Manel N, Garrido EN, Valle C, Sitbon M, Battini JL. HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding. Retrovirology. 2004 1(1):41.

Kim FJ, Manel N, Battini JL, Sitbon M. Envelope capture by retroviruses. Med Sci (Paris). 2004 20(10):876-81.

Manel N, Taylor N, Kinet S, Kim FJ, Swainson L, Lavanya M, Battini JL, Sitbon M. HTLV envelopes and their receptor GLUT1, the ubiquitous glucose transporter: a new vision on HTLV infection? Front Biosci. 2004 9:3218-41.

Sauce D, Mercier P, Battini JL, Ferrand C, Certoux JM, Manel N, Taylor N, Duperrier A, Herve P, Tiberghien P, Robinet E. Preferential retroviral-mediated transduction of EBV- and CMV-specific T cells after polyclonal T-cell activation. Gene Ther. 2004 11(12):1019-22.

Manel N, Kinet S, Kim FJ, Taylor N, Sitbon M, Battini JL. GLUT-1 is the receptor of retrovirus HTLV. Med Sci (Paris). 2004 20(3):277-9.

Goh WC, Manel N, Emerman M. The human immunodeficiency virus Vpr protein binds Cdc25C: implications for G2 arrest. Virology. 2004 318(1):337-49.

Kim FJ, Battini JL, Manel N, Sitbon M. Emergence of vertebrate retroviruses and envelope capture. Virology. 2004 318(1):183-91.

Manel N, Kim FJ, Kinet S, Taylor N, Sitbon M, Battini JL. The ubiquitous glucose transporter GLUT-1 is a receptor for HTLV. Cell. 2003 115(4):449-59.

Kim FJ, Manel N, Boublik Y, Battini JL, Sitbon M. Human T-cell leukemia virus type 1 envelope-mediated syncytium formation can be activated in resistant Mammalian cell lines by a carboxy-terminal truncation of the envelope cytoplasmic domain. J Virol. 2003 77(2):963-9.

Manel N, Kinet S, Battini JL, Kim FJ, Taylor N, Sitbon M. The HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis. Blood. 2003 101(5):1913-8.