How to request plasmids from the ron lab:

Updated: November 15, 2006

Dear Colleague,

We are happy to make certain items from our plasmid collection as well as certain worm and mouse strains available to qualified researchers.

The list of plasmids readily available from the lab, a brief description of their properties and often the full predicted sequence of the plasmid is available at the following link. In general we limit the number of plasmids shipped to any one lab to four or less. The worm strains listed on our website are available through the CGC.

After carefully choosing the plasmids you want, please fill in the online request form. Please copy the names of the plasmids you need exactly as they appear in the aforementioned web page. You will be asked to include a Fed-X account number to which shipping charges can be made(unfortunately, because of extensive package loss when using conventional air mail, we are unable to ship reagents without a valid Fed-X account number, or a credit card number allowing reversal of the shipping charges). You will also be asked to provide a brief description of the experiments you propose to conduct with the reagents you are requesting.

You will also be asked to confirm that you have read and agree with the terms of the "IMPORTANT NOTICE" below.

We typically deliver the plasmids as bacterial stabs within 2-4 weeks of receipt of your request.

Because of their irreplenishable nature, we are often unable to meet requests for antisera. For your benefit, we have complied a brief list of some sought-after reagents that are available commercially. Cell lines, though replenishable, are expensive to expand and ship and therefore we do not offer them on a routine basis. However, in exceptional circumstances we are willing to share cell lines and other more difficult-to-ship or irreplenishable reagents such as antisera with colleagues.

To request such reagents, please send a detailed letter to David Ron (if you haven't done so already). Once we have agreed on the terms of the transfer of these more cumbersome reagents, please visit our website to fill the online request form. You will also need to send a copy of our e-mail exchange describing the terms under which the reagents are to be provided to our staff, who will then try to fill your order.

You will receive a confirmatory e-mail.

Good luck with your research.

The Ron Lab

IMPORTANT NOTICE:

The reagents are being provided with the understanding that they will be used in academic research and will not be exploited commercially. While we make every effort to insure that the reagents you receive are the ones you requested, mistakes in handling, labeling and shipping do occur. Please beware that the reagents are being provided "as is" free of charge and with no warranty as to their quality, biological properties or suitability for any specific application.

While we do not, as a matter of policy, share information pertaining to your request with third parties, we are unable to guarantee the confidentiality of the information contained in your request and assume no liability for damages that may result from the disseminated of such information.

The Ron lab and NYU School of Medicine assumes no liability for damages incurred by use or misuse of these research reagents and it is the responsibility of the party receiving these reagents to be in compliance with all local and Federal rules and regulations pertaining to their use.

We also ask that you agree to abide, within reason, by the common etiquette governing the use of reagents received from other labs and inform us, before publication, of relevant results that were obtained using our reagent and could not be obtained otherwise. We also expect that you will seek and obtain permission from us before sharing these reagents or derivatives thereof with parties outside your lab.

We also expect that you will acknowledge our contribution of reagents in the printed version of any manuscript that describes their use. Usually, such an acknowledgement will appear in a special Acknowledgement Section or equivalent thereof (e.g. last reference in Science papers). Acknowledgment in the electronic supplements and methods section can also be made, but should not substitute for the above.

In some instances there might be a choice in the names you use to describe the reagents we have provided you. By accepting reagents from our lab, you undertake the obligation to use our nomenclature for the genes and proteins in question throughout any publication that describes their use. Thus GADD153 becomes CHOP, PEK is PERK and so on. In rare instances, journal policy may require that you use official gene names in which case PERK becomes EIF2AK3 and the PERK-/- cells line would be EIF2AK3-/- cells, but in such cases too, we expect you to make mention of our commonly used protein and gene name.