Please give the front sheet which contains the invoice to David or Rivka. The datasheet is yours. The oligonucleotides are shipped dry. The datasheet provides information on the yield of the syntesis, which serves as the basis for calculating the volume of water that each tube should be suspended in. As a general rule, oligonucleotides are solubilized at 100pM/mL (i.e. 100mM concentration)

We go to great lengths to insure that the original tube will be re-constituted in water that is free from any contaminating DNA. To this end please use the special PCR pipettemen, tips and special PCR water expressedly set aside for ththis purpose. Also, please work in an environment that is far removed from contaminating sources of cloned DNA. Contamination of the primers with cloned or PCR'd template DNA may render them useless to you and your colleagues.

After reconstitution in water, the original tube should be placed in a chronological oligonucelotide collection of which our lab has two: one dedicated to C. elegans and the other to all other species. Before stowing away this pristine stock solution, please remove a reasonable working aliquot for your own purposes. Please, do not retain the original tube in your own "private" collection, store it in its rightful place in the CHRONOLOGICAL COLLECTION. If you find original tubes anywhere else in the lab, please bring the matter to Rivka's attention and she will arrange for the tube(s) in question to be re-incorporated into the chronological collection at the appropiate position.

Please help us preserve this important resource

June 29, 2004