In the “Sequencing Archive” folder (in the Ron Lab) you will find an excel spread-sheet called “RonLab Sequence Log”. Please fill in an entry corresponding to each sequencing reaction you submit. The fields in that spread-sheet are intuitively obvious:

The “Date Submitted” refers to the date you gave the DNA for sequencing

Into the “Target” field, please input the name of the plasmid or PCR product you are analyzing. Please try to provide the information as accurately as possible. For example, if the plasmid you are sequencing corresponds to an entry in the “Seq.RonLab” folder, please use precisely the same name used there (including periods hyphens and the like); this will permit your colleagues to use the “finder” to establish correspondence between the plasmid and the sequence at a later date. The safest thing is to copy the GeneWorks file name and paste it in the “Target” field.

Similar considerations apply to the “Primer” field. Copy the primer’s name exactly as it appears in the “Oligos” folder in the Ron_Lab.

“Sequence ID” is the unique name assigned to your sequence by the facility. You will be able to fill out this field only after your sequence has been run.

“Comments”. Did the run work? Was it reasonably long. If you can, please write down the run’s length.

“Facility”. Who did the sequencing (NYU or another facility). If the sequencing was done by Seqwrite, you should consult a file called "Recovering data from SeqWright" in the "Protocols Lab" folder in the Ron_Lab.

Please place a copy of the I˚ data from the sequencing reaction in the archive folder. The name of the file you place in the “Sequencing Archive” folder should correspond precisely to the information in the “Sequence ID” field in the exel spreadsheet.  Please note the I˚ data is the file with the fluorescent tracing of the run (usually 200-300 kilobytes and ends with a suffix of ".ab1"), not the string of letters inferred from it. The latter text file (which is much smaller, usually about 8 kilobytes, and ends with the suffix ".seq") should not be archived as it can be readily derived from the I˚ data.

Finally, we would now ask that you create an entry in the excel spread sheet for your sequencing project BEFORE you send the plasmids out for sequencing and later update the entry once the results are in. Miho has been asked to ascertain that an entry has been created for every sequencing project before she sends it out

May 12, 2006