ID   Fv2E-PERK.pBABEpu; DNA; 7521 BP.                                         
XX                                                                             
AC   Citation: Lu, P. D., Jousse, C., Marciniak, S. J., Zhang, Y., Novoa, I.
AC   Scheuner, D., Kaufman, R. J., Ron, D., and Harding, H. P.
AC   (2004). Cytoprotection by pre-emptive conditional phosphorylation of 
AC   translation initiation factor 2. Embo J 23, 169-179
XX                                                                             
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
XX                                                                             
DE   pZOME-1-C                                                                 
XX                                                                             
KW   .                                                                         
XX                                                                             
OS   Unknown                                                                   
XX                                                                             
OC   Unclassified.                                                             
XX                                                                             
RN   [1](bases 1 to 5602)                                                      
RA   EUROSCARF                                                                 
RL   http://                                                                   
XX                                                                             
CC   SOURCE      Unknown. Insertion of cDNA into the BamHI site at             
CC   Position 1344 (pZome1C contains two NcoI sites): GGA TCC ATG              
CC   GAA...//Typed 30 residues at position 1348 Deleted residues 1378          
CC   to 1379 Deleted residues 1912 to 1930 and replaced it with                
CC   residues 1912 to 1913 Inserted 75 residues (Residues 83 to 158 of         
CC   pBABEPu.PL) at position 1310replacing residues 1310 to 1914               
CC   Inserted 2453 residues (Residues 3013 to 5466 of F                        
CC   v2E-mPerk2.9E10.V2.pCDNA1/AMP) at position 1368replacing residues         
CC   1368 to 1385                                                              
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   CDS             1388..3817                                                
FT                   /note="Fv2E+PERK.KD+9E10                                  
FT                   /codon_start=1"                                           
FT   CDS             complement(7374..6514)                                    
FT                   /gene="AmpR"/product="beta lactamase"                     
FT   conflict        complement(2431..2431)                                    
FT                   /note="N in .3Bam230@T3"                                  
FT   conflict        2542                                                      
FT                   /note=""y""                                               
FT   conflict        2563                                                      
FT                   /note=""y""                                               
FT   conflict        2834                                                      
FT                   /note=""W", A>T"                                          
FT   conflict        3029                                                      
FT                   /note=""T" in .3Pst1500@T3"                               
FT   insertion_seq   2049..2060                                                
FT                   /note="linker insertion to get sal"                       
FT   LTR             1..460                                                    
FT                   /gene="5'                                                 
FT                   LTR"/note="The left LTR serves as the promoter of         
FT                    the subcloned                                            
FT   LTR             4833..5458                                                
FT                   /gene="3'                                                 
FT                   LTR"/product="3' LTR"                                     
FT   misc_binding    2300..2302                                                
FT                   /note="ATP binding"                                       
FT   misc_feature    2583..2993                                                
FT                   /note="AA067988"                                          
FT   misc_feature    2920..3380                                                
FT                   /note="AA217773"                                          
FT   misc_feature    3156..3616                                                
FT                   /note=".3Pst1100@T3"                                      
FT   misc_feature    3356..3821                                                
FT                   /note="peri.Sma.seq"                                      
FT   misc_feature    3595..3821                                                
FT                   /note=".3.dXba@T7"                                        
FT   misc_feature    3692..3786                                                
FT                   /note="peri.Sma.seq"                                      
FT   misc_feature    3692..3786                                                
FT                   /note=".3.dXba@T7"                                        
FT   misc_feature    3787..3821                                                
FT                   /note="9E10-Xho added"                                    
FT   misc_feature    5755..5774                                                
FT                   /gene="Origin"/product="origin of replcation"             
FT   primer          1468..1488                                                
FT                   /note="FKBP.2AS"                                          
FT   primer          3797..3821                                                
FT                   /note="PERK.STOP>Nru1"                                    
FT   primer          3877..3900                                                
FT                   /note="pBABE.2AS"                                         
FT   primer          1311..1334                                                
FT                   /note="BABEP.1S"                                          
FT   promoter        3829..4171                                                
FT                   /gene="SV 40 early                                        
FT                   promoter"/product="SV 40 early promoter"                  
FT   CDS             4176..4775                                                
FT                   /gene="Puro-R"/product="puromycin N-acetyltransfe         
FT                   rase"                                                     
FT                   /codon_start=1                                            
FT   misc_feature    920..1309                                                 
FT                   /gene="gag"/note="the start codon of the gag gene         
FT                    has been mutated sothat it does not get                  
FT                   expressed. Its presence, however,increases the            
FT                   level of retroviral production"                           
FT   primer          2056..2078                                                
FT                   /note="PERK2.Sal.1S"                                      
FT   primer          2282..2313                                                
FT                   /note="PERK2.(K/A).1"                                     
FT   primer          2630..2653                                                
FT                   /note="PERK.2410AS"                                       
FT   primer          3769..3785                                                
FT                   /note="PERK2.9E10.1"                                      
FT   primer          3810..3821                                                
FT                   /note="PERK2.9E10.1"                                      
XX                                                                             
SQ   Sequence   7521 BP;  1776 A; 2111 C; 1957 G; 1676 T;    1 Other;          
     CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA         
     CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC         
     CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA         
     TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC         
     AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC         
     AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC         
     CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC         
     CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG         
     GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA         
     TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA         
     GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC         
     CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG         
     AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA         
     GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG         
     CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT         
     TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG         
     TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG         
     TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG         
     CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA         
     TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC         
     TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC         
     GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT         
     CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACGTAGAA TTCCAGAAGC         
     CGCCACCATG GCTTCTAGAG GCGTCCAAGT CGAAACCATT AGTCCCGGCG ATGGCAGAAC         
     ATTTCCTAAA AGGGGACAAA CATGTGTCGT CCATTATACA GGCATGTTGG AGGACGGCAA         
     AAAGGTGGAC AGTAGTAGAG ATCGCAATAA ACCTTTCAAA TTCATGTTGG GAAAACAAGA         
     AGTCATTAGG GGATGGGAGG AGGGCGTGGC TCAAATGTCC GTCGGCCAAC GCGCTAAGCT         
     CACCATCAGC CCCGACTACG CATACGGCGC TACCGGACAT CCCGGAATTA TTCCCCCTCA         
     CGCTACCTTG GTGTTTGACG TCGAACTGTT GAAGCTCGAG ACTAGAGGAG TGCAGGTGGA         
     GACTATCTCC CCAGGAGACG GGCGCACCTT CCCCAAGCGC GGCCAGACCT GCGTGGTGCA         
     CTACACCGGG ATGCTTGAAG ATGGAAAGAA AGTTGATTCC TCCCGGGACA GAAACAAGCC         
     CTTTAAGTTT ATGCTAGGCA AGCAGGAGGT GATCCGAGGC TGGGAAGAAG GGGTTGCCCA         
     GATGAGTGTG GGTCAGAGAG CCAAACTGAC TATATCTCCA GATTATGCCT ATGGTGCCAC         
     TGGGCACCCA GGCATCATCC CACCACATGC CACTCTCGTC TTCGATGTGG AGCTTCTAAA         
     ACTGGAAACT AGGGGTCGAC GGCTTTTCCA TCCTCAGCCC CACAGGCAGC GGAAGGAGTC         
     TGAAACTCAG TGCCAGACTG AAAGTAAATA CGACTCCGTG AGTGCCGATG TCAGTGACAA         
     CAGCTGGAAT GACATGAAGT ACTCAGGATA CGTATCCCGA TATCTAACAG ATTTTGAGCC         
     AATTCAGTGC ATGGGTCGTG GTGGCTTTGG CGTTGTCTTT GAAGCTAAAA ACAAAGTAGA         
     TGACTGCAAT TACGCTATCA AGAGGATCCG GCTCCCCAAC AGGGAGTTGG CACGGGAGAA         
     GGTAATGCGG GAAGTTAAAG CCTTGGCTAA GCTGGAACAC CCAGGCATTG TGAGGTATTT         
     CAACGCCTGG CTGGAAACCC CACCAGAGAA GTGGCAAGAA GAGATGGATG AGATCTGGCT         
     CAAAGACGAA AGCACAGACT GGCCGCTCAG CTCCCCTAGC CCGATGGATG CCCCATCTGT         
     TAAGATCCGA AGGATGGATC CYTTCTCTAC AAAAGAGCAG ATCGAAGTCA TAGCTCCTTC         
     TCCTGAAAGA AGTCGGTCTT TCTCGGTGGG CATTTCCTGT GGCCAGACAA GCTCATCGGA         
     GAGCCAGTTC TCTCCCCTGG AGTTCTCAGG GACAGACTGC GGAGACAACA GTGACTCAGC         
     GGACGCAGCC TACAACCTCC AGGACAGTTG CCTGACGGAC TGCGAGGACG TGGAAGATGG         
     CACCGTGGAC GGCAATGACG AGGGACACTC CTTTGAACTT TGTCCGTCCG AAGCTTCTCC         
     CTATACCCGG TCTAGGGAAG GAACGTCCTC CTCCATAGTG TTTGAGGACT CTGGCTGCGG         
     CAACGCGTCC AGTAAGGAGG AGCCCAGAGG GAACCGGCTG CATGATGGCA ACCATTATGT         
     TAATAAGCTA ACTGATCTCA AGTGCTCCAG CAGCAGGTCT TCTTCAGAAG CCACCACCTT         
     GTCTACCTCC CCTACCAGGC CAACCACTCT AAGCTTGGAT TTCACCAAGA ACACTGTGGG         
     CCAGCTCCAG CCCAGCTCCC CCAAGGTGTA TCTGTACATT CAGATGCAGC TGTGCAGGAA         
     GGAGAACCTC AAAGACTGGA TGAACCGGCG CTGCAGCTTG GAGGACCGGG AGCACGGCGT         
     GTGCCTGCAC ATCTTCCTGC AGATCGCAGA GGCAGTGGAG TTCCTGCACA GCAAGGGACT         
     CATGCACAGG GACCTCAAGC CTTCCAACAT ATTCTTCACA ATGGATGATG TGGTCAAGGT         
     TGGGGACTTT GGACTGGTGA CTGCTATGGA CCAAGATGAA GAAGAGCAGA CTGTTCTGAC         
     TCCAATGCCA GCCTATGCTA CGCACACGGG ACAAGTAGGG ACCAAGCTAT ACATGAGCCC         
     AGAGCAGATT CATGGAAACA ACTACTCCCA TAAAGTGGAC ATCTTCTCTT TAGGCTTGAT         
     TCTGTTTGAA CTCCTCTACC CATTCAGCAC CCAGATGGAA CGAGTCCGGA TTTTAACTGA         
     TGTCAGAAAT CTCAAGTTTC CTCTACTGTT CACTCAGAAA TATCCCCAAG AGCATATGAT         
     GGTTCAAGAC ATGCTCTCTC CATCCCCCAC GGAGCGGCCT GAAGCCACAG ACATCATTGA         
     AAATGCCATA TTTGAGAACT TGGAGTTTCC CGGGAAAACG GTTCTGAGAC AGCGGTCCCG         
     CTCCATGAGT TCATCTGGAA CAAAACATTC CAGACAGCCC AGCTGCTCGT ACACGCCACT         
     GCCTGGCGAG CAGAAGCTGA TCTCTGAGGA GGATCTGTGA CTCGACCCTG TGGAATGTGT         
     GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC         
     ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA         
     TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC         
     CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA         
     TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT         
     TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC         
     GCCTCGCCAC CCGCGACGAC GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG         
     ACTACCCCGC CACGCGCCAC ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC         
     TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG         
     ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG         
     CCGAGATCGG CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA         
     TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG         
     GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG         
     AGGCGGCCGA GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC         
     CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC         
     GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC         
     CGACCGAAAG GAGCGCACGA CCCCATGCAT CGATAAAATA AAAGATTTTA TTTAGTCTCC         
     AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC         
     CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG         
     GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC         
     CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG         
     TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC         
     CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC         
     CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG         
     CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG         
     ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT         
     GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC         
     TTTCACATGC AGCATGTATC AAAATTAATT TGGTTTTTTT TCTTAAGTAT TTACATTAAA         
     TGGCCATAGT TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG         
     CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG         
     GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA         
     AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG         
     GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG         
     AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC         
     GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG         
     GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT         
     CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC         
     GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC         
     ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG         
     TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA         
     GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC         
     GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT         
     CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT         
     TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTGCGGCC GCAAATCAAT         
     CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC         
     TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT         
     AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC         
     ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG         
     AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG         
     AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT         
     GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG         
     AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT         
     TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC         
     TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC         
     ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA         
     TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG         
     AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC         
     CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG         
     GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT         
     CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT         
     TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC         
     ACCTGCAGCC TGAATATGGG C                                                   
//