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GST-tagged wildtype mouse GCN2 kinase domain in pGEX4T-1 Used to raise antisera to GCN2. Described in: Harding, H, Novoa, I, Zhang, Y, Zeng, H, Schapira, M, and Ron, D. 2000. Regulated translation initiation controls stress-induced gene expression in mammalian cells. Molecular Cell. 6: 1099-1108 Supplied as a stab of E. coli (strain unknown) transformed with the ampicillin resistant plasmid, GCN2-KD1-pGEX4T-1. The predicted sequence of the plasmid is provide in EMBL format. |
