ID   pZOME-1-C standard; DNA; 6721 BP.                                         
XX                                                                             
AC   pZOME-1-C                                                                 
AC   pZOME-1-C                                                                 
AC   pZOME-1-C                                                                 
AC   pZOME-1-C                                                                 
XX                                                                             
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
DT   20-FEB-2003                                                               
XX                                                                             
DE   pZOME-1-C                                                                 
XX                                                                             
KW   .                                                                         
XX                                                                             
OS   Unknown                                                                   
XX                                                                             
OC   Unclassified.                                                             
XX                                                                             
RN   [1](bases 1 to 5602)                                                      
RA   EUROSCARF                                                                 
RL   http://                                                                   
XX                                                                             
CC   SOURCE      Unknown. Insertion of cDNA into the BamHI site at             
CC   Position 1344 (pZome1C contains two NcoI sites): GGA TCC ATG              
CC   GAA...//Typed 30 residues at position 1348 Deleted residues 1378          
CC   to 1379 Deleted residues 1912 to 1930 and replaced it with                
CC   residues 1912 to 1913 Inserted 75 residues (Residues 83 to 158 of         
CC   pBABEPu.PL) at position 1310replacing residues 1310 to 1914               
CC   Inserted 1636 residues (Residues 904 to 2540 of Myd116 pBS) at            
CC   position 1364                                                             
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   LTR             1..460                                                    
FT                   /gene="5'                                                 
FT                   LTR"/note="The left LTR serves as the promoter of         
FT                    the subcloned                                            
FT   misc_feature    920..1309                                                 
FT                   /gene="gag"/note="the start codon of the gag gene         
FT                    has been mutated sothat it does not get                  
FT                   expressed. Its presence, however,increases the            
FT                   level of retroviral production"                           
FT   primer          1311..1334                                                
FT                   /note="BABEP.1S"                                          
FT   CDS             1395..3000                                                
FT                   /note="GADD34 CDS                                         
FT                   /codon_start=1"                                           
FT   CDS             1395..3311                                                
FT                   /note="CDS                                                
FT                   /codon_start=1"                                           
FT   allele          2235..2351                                                
FT                   /note="First 38-aa sequence repeat"                       
FT   allele          2352..2465                                                
FT                   /note="Second 38-aa sequence repeat"                      
FT   allele          2466..2579                                                
FT                   /note="Third 38-aa sequence repeat"                       
FT   allele          2580..2693                                                
FT                   /note="Fourth 38-aa sequence repeat"                      
FT   allele          2694..2774                                                
FT                   /note="Fifth 38-aa sequence repeat (only 27 aa)"          
FT   primer          2816..2835                                                
FT                   /note="mMYD116.Bam.3S"                                    
FT   promoter        3029..3371                                                
FT                   /gene="SV 40 early                                        
FT                   promoter"/product="SV 40 early promoter"                  
FT   primer          3077..3100                                                
FT                   /note="pBABE.2AS"                                         
FT   CDS             3376..3975                                                
FT                   /gene="Puro-R"/product="puromycin N-acetyltransfe         
FT                   rase"                                                     
FT                   /codon_start=1                                            
FT   LTR             4033..4658                                                
FT                   /gene="3'                                                 
FT                   LTR"/product="3' LTR"                                     
FT   misc_feature    4955..4974                                                
FT                   /gene="Origin"/product="origin of replcation"             
FT   CDS             complement(6574..5714)                                    
FT                   /gene="AmpR"/product="beta lactamase"                     
XX                                                                             
SQ   Sequence   6721 BP;  1574 A; 1924 C; 1792 G; 1431 T;                      
     CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA         
     CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC         
     CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA         
     TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC         
     AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC         
     AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC         
     CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC         
     CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG         
     GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA         
     TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA         
     GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC         
     CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG         
     AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA         
     GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG         
     CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT         
     TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG         
     TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG         
     TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG         
     CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA         
     TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC         
     TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC         
     GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT         
     CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACGGGGTG ACGTGCAGCC         
     CGCCGCCCAG ACACATGGCC CCGAGCCCAA GACCCCAGCA TGTCCTGCAC TGGAGGGACG         
     CCCACAACTT CTATCTCCTG TCCCCACTGA TGGGCTTGCT CAGTCGGGCC TGGAGCCGCC         
     TGAGGGGCCC AGAAGTCCCA GAGGCATGGC TGGCAAAAAC AGTAACAGGA GCAGATCAGA         
     TAGAAGCTGC GGCTCTGCTG ACACCTACCC CTGTCTCTGG TAACCTCCTC CCTCATGGGG         
     AGACTGAAGA AAGTGGATCT CCTGAACAGA GTCAAGCAGC CCAGAGGCTC TGCCTTGTGG         
     AAGCTGAAAG TTCCCCTCCT GAAACTTGGG GACTTTCAAA TGTTGATGAG TACAATGCAA         
     AGCCAGGACA AGATGACCTT AGAGAGAAGG AAATGGAACG CACAGCTGGC AAGGCCACAC         
     TACAGCCCGC TGGCCTGCAA GGGGCTGATA AGAGGCTTGG GGAGGTGGTG GCTAGAGAAG         
     AGGGAGTGGC TGAGCCCGCT TATCCCACAT CACAGCTGGA GGGTGGTCCA GCTGAGAATG         
     AAGAGGATGG AGAAACAGTG AAGACTTACC AAGCTTCTGC TGCTTCCATA GCTCCGGGAT         
     ACAAACCCAG CACCCCTGTG CCTTTCTTGG GGGAGGCAGA ACATCAAGCC ACGGAAGAAA         
     AAGGAACAGA AAACAAGGCT GACCCCTCCA ACTCTCCTTC TTCAGGCTCC CACTCCAGAG         
     CCTGGGAGTA CTACTCTAGA GAGAAGCCTA AGCAGGAGGG AGAAGCCAAG GTAGAGGCAC         
     ACAGGGCAGG GCAGGGTCAC CCTTGTCGGA ATGCTGAGGC TGAGGAAGGA GGACCTGAGA         
     CAACTTTTGT CTGTACTGGA AATGCCTTCC TGAAGGCCTG GGTGTATCGC CCAGGAGAGG         
     ACACAGAGGA AGAAGACAAC AGCGATTCGG ATTCAGCTGA GGAAGACACA GCTCAGACCG         
     GTGCCACCCC CCATACAAGT GCCTTCCTGA AGGCCTGGGT GTATCGCCCA GGAGAGGACA         
     CAGAGGAAGA AGACAGCGAT TCGGATTCAG CTGAGGAAGA CACAGCTCAG ACCGGTGCCA         
     CCCCCCATAC AAGTGCCTTC CTGAAGGCCT GGGTGTATCG CCCAGGAGAG GACACAGAGG         
     AAGAAAACAG CGATTTGGAT TCAGCTGAGG AAGACACAGC TCAGACCGGT GCCACCCCCC         
     ATACAAGTGC CTTCCTGAAG GCCTGGGTGT ATCGCCCAGG AGAGGACACA GAGGAAGAAA         
     ACAGCGATTT GGATTCAGCT GAGGAAGACA CAGCTCAGAC CGGTGCCACC CCACATACAA         
     GTCCCTTCCT GAAGGCCTGG GTGTATCGCC CAGGAGAGGA CACAGAAGAT GACACAGAAG         
     AGGAAGAGGA CAGTGAGAAT GTGGCCCCAG GTGACTCAGA AACAGCTGAC TCAAGCCAGA         
     GTCCCTGCCT TCAGCCCCAG CGTTGTCTAC CAGGAGAGAA GACCAAGGGA CGTGGGGAAG         
     AGCCCCCTCT CTTCCAGGTG GCCTTCTATT TACCCGGAGA GAAGCCAGAA TCACCTTGGG         
     CTGCACCTAA GCTGCCCCTT CGACTGCAGA GGCGGCTCAG ATTGTTCAAA GCCCCCACCC         
     GTAGAATTCC CAGCACAGTG GTCGACCCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG         
     TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC         
     AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT         
     TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT         
     TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC         
     GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT         
     TGCAAAAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC         
     GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC         
     ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG         
     CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC         
     TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG         
     GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG         
     CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG         
     GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG         
     GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC         
     TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC         
     AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA         
     CCCCATGCAT CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA         
     GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA         
     AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA         
     TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA         
     GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC         
     TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC         
     CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA         
     ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG         
     AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC         
     GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA         
     GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCACATGC AGCATGTATC         
     AAAATTAATT TGGTTTTTTT TCTTAAGTAT TTACATTAAA TGGCCATAGT TGCATTAATG         
     AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT         
     CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC         
     GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG         
     CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG         
     CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG         
     ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC         
     CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA         
     TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT         
     GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC         
     CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG         
     AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC         
     TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT         
     TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA         
     GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG         
     GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA         
     AAGGATCTTC ACCTAGATCC TTTTGCGGCC GCAAATCAAT CTAAAGTATA TATGAGTAAA         
     CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT         
     TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT         
     TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT         
     TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT         
     CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA         
     ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG         
     GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT         
     TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG         
     CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG         
     TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC         
     GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA         
     CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC         
     CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT         
     TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG         
     GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA         
     GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA         
     AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGCAGCC TGAATATGGG         
     C                                                                         
//