FLAG-epitope tagged MyD116 (GADD34) in the mammalian expression plasmid pFLAG-CMV-2 (see: · Novoa, I, Zeng, H, Harding, HP, and Ron D. 2001. Feedback inhibition of the unfolded protein response by GADD34-mediated dephosphorylation of eIF2a. J Cell Biol 153:1011-1021)
MyD116.PFLAG.CMV2 (6749nt, predicted).
CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTGAT CTAATCAATA TTGGCCATTA GCCATATTAT TCATTGGTTA TATAGCATAA ATCAATATTG GCTATTGGCC ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CGACCGCCCA GCGACCCCCG CCCGTTGACG TCAATAGTGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT AGCATTATGC CCAGTACATG ACCTTACGGG AGTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGAATTGATC TACCATGGAC TACAAAGACG ATGACGACAA GCTTGCGGCC GGGGTGACGT GCAGCCCGCC GCCCAGACAC ATGGCCCCGA GCCCAAGACC CCAGCATGTC CTGCACTGGA GGGACGCCCA CAACTTCTAT CTCCTGTCCC CACTGATGGG CTTGCTCAGT CGGGCCTGGA GCCGCCTGAG GGGCCCAGAA GTCCCAGAGG CATGGCTGGC AAAAACAGTA ACAGGAGCAG ATCAGATAGA AGCTGCGGCT CTGCTGACAC CTACCCCTGT CTCTGGTAAC CTCCTCCCTC ATGGGGAGAC TGAAGAAAGT GGATCTCCTG AACAGAGTCA AGCAGCCCAG AGGCTCTGCC TTGTGGAAGC TGAAAGTTCC CCTCCTGAAA CTTGGGGACT TTCAAATGTT GATGAGTACA ATGCAAAGCC AGGACAAGAT GACCTTAGAG AGAAGGAAAT GGAACGCACA GCTGGCAAGG CCACACTACA GCCCGCTGGC CTGCAAGGGG CTGATAAGAG GCTTGGGGAG GTGGTGGCTA GAGAAGAGGG AGTGGCTGAG CCCGCTTATC CCACATCACA GCTGGAGGGT GGTCCAGCTG AGAATGAAGA GGATGGAGAA ACAGTGAAGA CTTACCAAGC TTCTGCTGCT TCCATAGCTC CGGGATACAA ACCCAGCACC CCTGTGCCTT TCTTGGGGGA GGCAGAACAT CAAGCCACGG AAGAAAAAGG AACAGAAAAC AAGGCTGACC CCTCCAACTC TCCTTCTTCA GGCTCCCACT CCAGAGCCTG GGAGTACTAC TCTAGAGAGA AGCCTAAGCA GGAGGGAGAA GCCAAGGTAG AGGCACACAG GGCAGGGCAG GGTCACCCTT GTCGGAATGC TGAGGCTGAG GAAGGAGGAC CTGAGACAAC TTTTGTCTGT ACTGGAAATG CCTTCCTGAA GGCCTGGGTG TATCGCCCAG GAGAGGACAC AGAGGAAGAA GACAACAGCG ATTCGGATTC AGCTGAGGAA GACACAGCTC AGACCGGTGC CACCCCCCAT ACAAGTGCCT TCCTGAAGGC CTGGGTGTAT CGCCCAGGAG AGGACACAGA GGAAGAAGAC AGCGATTCGG ATTCAGCTGA GGAAGACACA GCTCAGACCG GTGCCACCCC CCATACAAGT GCCTTCCTGA AGGCCTGGGT GTATCGCCCA GGAGAGGACA CAGAGGAAGA AAACAGCGAT TTGGATTCAG CTGAGGAAGA CACAGCTCAG ACCGGTGCCA CCCCCCATAC AAGTGCCTTC CTGAAGGCCT GGGTGTATCG CCCAGGAGAG GACACAGAGG AAGAAAACAG CGATTTGGAT TCAGCTGAGG AAGACACAGC TCAGACCGGT GCCACCCCAC ATACAAGTCC CTTCCTGAAG GCCTGGGTGT ATCGCCCAGG AGAGGACACA GAAGATGACA CAGAAGAGGA AGAGGACAGT GAGAATGTGG CCCCAGGTGA CTCAGAAACA GCTGACTCAA GCCAGAGTCC CTGCCTTCAG CCCCAGCGTT GTCTACCAGG AGAGAAGACC AAGGGACGTG GGGAAGAGCC CCCTCTCTTC CAGGTGGCCT TCTATTTACC CGGAGAGAAG CCAGAATCAC CTTGGGCTGC ACCTAAGCTG CCCCTTCGAC TGCAGAGGCG GCTCAGATTG TTCAAAGCCC CCACCCGGGA TCAGGACCCC GAGATTCCTC TAAAAGCTCG GAAGGTACAC TTCGCTGAGA AAGTCACAGT CCATTTCCTT GCTGTCTGGG CAGGACCAGC CCAAGCTGCC CGTCGAGGTC CCTGGGAGCA GTTTGCACGA GATCGAAGCC GCTTTGCTCG ACGCATTGCC CAGGCAGAGG AGAAGCTGGG TCCCTACCTT ACCCCTGATT CCAGGGCCAG AGCATGGGCA CGCCTTAGAA ACCCATCTCT TCCACAGTCC GAGCCTCGCT CTTCCTCTGA GGCCACTCCC TTGACCCAAG ATGTGACCAC ACCCTCTCCC CTTCCCAGTG AAACCCCTTC GCCCAGCCTG TACTTGGGAG GGAGGCGGGG CTAAGCCTGA GTAGTTTCCT ATTATTTATT TATTTATTTA TTTGAATAAG AAATAAAGCC TTTTAATTTG TAGTGATGAA TTCATCGATA GATCTGATAT CGGTACCAGT CGACTCTAGA GGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCT TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCCCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTCGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGCCATGG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCTTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCTCGA GGAACTGAAA AACCAGAAAG TTAATTCCCT ATAGTGAGTC GTATTAAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAAACGT TTACAATTT