ID   pZOME-1-C standard; DNA; 6702 BP.                                         
XX                                                                             
AC   pZOME-1-C                                                                 
XX                                                                             
DT   20-FEB-2003                                                               
XX                                                                             
DE   pZOME-1-C                                                                 
XX                                                                             
KW   .                                                                         
XX                                                                             
OS   Unknown                                                                   
XX                                                                             
OC   Unclassified.                                                             
XX                                                                             
RN   [1](bases 1 to 5602)                                                      
RA   EUROSCARF                                                                 
RL   http://                                                                   
XX                                                                             
CC   SOURCE      Unknown. Insertion of cDNA into the BamHI site at             
CC   Position 1344 (pZome1C contains two NcoI sites): GGA TCC ATG              
CC   GAA...//Deleted residues 1310 to 1981 Inserted 154 residues               
CC   (Residues 85 to 239 of pBabepuro partial) at position 1310 Deleted        
CC   residues 1364 to 1385 Inserted 1638 residues (Residues 769 to 2407        
CC   of boP58-pCMV-HA) at position 1364                                        
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   promoter        1310..3352                                                
FT                   /gene="SV 40 early                                        
FT                   promoter"/product="SV 40 early promoter"                  
FT   allele          3008..3081                                                
FT                   /note="SV40 early promoter"                               
FT   CDS             complement(6555..5695)                                    
FT                   /gene="AmpR"/product="beta lactamase"                     
FT   primer_bind     2966..3001                                                
FT                   /note="3'-(Bgl II)"                                       
FT   misc_feature    920..1309                                                 
FT                   /gene="gag"/note="the start codon of the gag gene         
FT                    has been mutated sothat it does not get                  
FT                   expressed. Its presence, however,increases the            
FT                   level of retroviral production"                           
FT   primer          3058..3081                                                
FT                   /note="Babe.2AS"                                          
FT   allele          1311..1343                                                
FT                   /note="GAG"                                               
FT   misc_feature    4936..4955                                                
FT                   /gene="Origin"/product="origin of replcation"             
FT   primer          1311..1334                                                
FT                   /note="Babe.1S"                                           
FT   LTR             4014..4639                                                
FT                   /gene="3'                                                 
FT                   LTR"/product="3' LTR"                                     
FT   CDS             1481..2995                                                
FT                   /codon_start=1                                            
FT   LTR             1..460                                                    
FT                   /gene="5'                                                 
FT                   LTR"/note="The left LTR serves as the promoter of         
FT                    the subcloned                                            
FT   primer_bind     1474..1499                                                
FT                   /note="5'-(EcoRI)"                                        
XX                                                                             
SQ   Sequence   6702 BP;  1661 A; 1790 C; 1699 G; 1552 T;                      
     CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA         
     CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC         
     CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA         
     TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC         
     AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC         
     AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC         
     CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC         
     CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG         
     GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA         
     TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA         
     GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC         
     CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG         
     AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA         
     GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG         
     CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT         
     TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG         
     TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG         
     TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG         
     CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA         
     TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC         
     TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC         
     GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT         
     CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACCCTGTA CGGAAGTGTT         
     ACTTCTGCTC TAAAAGCTGC GGAATTGTAC CCGCGGGCCC ACCATGTACC CATACGATGT         
     TCCAGATTAC GCTCTTATGG CCATGGAGGC CCGAATTCGG ATGGTCGCCC CCGGCTCTGT         
     GACCAGCCGG CTGGGCTCGG TGTTCCCTTT CCTGCTGGTC CTGGTGGACC TGCAGTACGA         
     AGGTGCTGAA TGTGGAGTAA ATGCAGATGT TGAGAAGCAT CTGGAATTGG GCAAGAAGCT         
     GCTCGCAGCC GGACAGCTCG CGGATGCGTT ATCTCAGTTT CACGCTGCAG TAGATGGTGA         
     CCCTGATAAC TATATTGCTT ACTATCGGAG AGCTACTGTC TTTTTAGCTA TGGGCAAATC         
     AAAAGCAGCA CTTCCTGATT TAACTAAAGT GATTGAATTG AAGATGGATT TCACTGCAGC         
     AAGATTACAG AGAGGTCACT TATTACTCAA ACAAGGAAAA CTTGATGAAG CAGAAGATGA         
     TTTTAAAAAA GTGCTCAAGT CAAATCCAAG TGAAAATGAA GAGAAGGAGG CCCAGTCCCA         
     GCTTGTCAAA TCTGATGAAA TGCAGCGTCT GCGCTCACAA GCACTGGATG CCTTTGAGAG         
     CTCAGATTTT ACTGCTGCTA TAACCTTCCT TGATAAGATT TTAGAGGTTT GTGTTTGGGA         
     TGCAGAACTT CGAGAACTTC GAGCTGAATG TTTTATAAAA GAAGGGGAAC CTAGGAAAGC         
     GATAAGTGAC TTAAAAGCTT CATCAAAATT GAAAAACGAT AATACTGAGG CATTTTATAA         
     AATCAGCACA CTCTACTATG AACTAGGAGA CCATGAACTG TCTCTCAGTG AAGTTCGTGA         
     ATGTCTTAAA CTTGACCAGG ATCATAAAAG GTGTTTTGCA CACTATAAAC AAGTAAAGAA         
     ACTGAATAAG CTGATTGAGT CAGCTGAAGA GCTCATCAAA GAAGGCAGGT ACACAGATGC         
     AATCAGCAAA TATGAATCTG TCATGAAAAC AGAGCCAGGT GTTCATGAAT ATACAATTCG         
     TTCAAAAGAA AGGATTTGCC ACTGCTTTTC TAAGGATGAG AAGCCTGTTG AAGCTATTCG         
     AGTATGTTCA GAAGTTTTAC AGGTGGAACC TGACAACGTG AATGCTCTGA AAGACCGAGC         
     AGAGGCCTAT TTAATAGAAG AAATGTATGA TGAAGCTATT CAGGATTATG AAACTGCTCA         
     GGAACACAAT GAGAATGATC AGCAGATTCG AGAAGGTCTG GAGAAAGCAC AGAGGCTACT         
     GAAACAGTCA CAGAGACGAG ATTATTACAA AATCTTGGGA GTAAAAAGAA ATGCCAAAAA         
     GCAAGAAATC ATTAAAGCAT ACCGAAAATT AGCACTGCAG TGGCACCCAG ACAACTTCCA         
     GAACGAAGAA GAAAAGAAAA AAGCTGAGAA GAAGTTCATT GACATAGCAG CTGCTAAAGA         
     AGTCCTCTCC GATCCAGAAA TGAGGAAGAA GTTTGATGAC GGAGAAGACC CCCTGGACGC         
     AGAGAGCCAA CAAGGAGGTG GCGGCAACCC TTTCCACAGG AGCTGGAACT CATGGCAAGG         
     GTTCAGTCCC TTTAGCTCAG GCGGACCTTT TAGATTTAAA TTCCACTTCA ATTAAAGATC         
     TCTCGACCCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG         
     GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG         
     GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC         
     CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC         
     ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT         
     TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTTACCATGA         
     CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG GCCGTACGCA         
     CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT CCGGACCGCC         
     ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG         
     GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG         
     TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC         
     GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG         
     CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG         
     CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA         
     CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG         
     TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC         
     CGCCCCACGA CCCGCAGCGC CCGACCGAAA GGAGCGCACG ACCCCATGCA TCGATAAAAT         
     AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG         
     GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG         
     AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT         
     CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGAATATG         
     GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG         
     GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA CCATCAGATG TTTCCAGGGT         
     GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT AACCAATCAG TTCGCTTCTC         
     GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA GAGCCCACAA CCCCTCACTC         
     GGGGCGCCAG TCCTCCGATT GACTGAGTCG CCCGGGTACC CGTGTATCCA ATAAACCCTC         
     TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG AGGGTCTCCT CTGAGTGATT         
     GACTACCCGT CAGCGGGGGT CTTTCACATG CAGCATGTAT CAAAATTAAT TTGGTTTTTT         
     TTCTTAAGTA TTTACATTAA ATGGCCATAG TTGCATTAAT GAATCGGCCA ACGCGCGGGG         
     AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG         
     GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA         
     GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC         
     CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC         
     AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG         
     TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC         
     CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT         
     CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG         
     CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC         
     TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT         
     GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT         
     ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC         
     AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA         
     AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC         
     GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC         
     CTTTTGCGGC CGCAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA         
     ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC         
     CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC         
     TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC         
     AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT         
     TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT         
     TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC         
     CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG         
     CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT         
     TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC         
     TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG         
     CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT         
     TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC         
     GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC         
     TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA         
     ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG         
     TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG         
     CACATTTCCC CGAAAAGTGC CACCTGCAGC CTGAATATGG GC                            
//