ID   Untitled  standard; circular DNA; 7232 BP.                                
XX                                                                             
XX                                                                             
DT   15-JUL-1996                                                               
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
CC   Inserted 5432 residues at position 0 Inserted 5446 residues at            
CC   position 0replacing residues 0 to 5432 Inserted 1850 residues             
CC   (Residues 930 to 2780 of mP58.FL1.pGEX-4T-1) at position                  
CC   907replacing residues 907 to 971                                          
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   misc_feature    1048..2562                                                
FT                   /note="P58 CDS"                                           
FT   misc_feature    2221..2439                                                
FT                   /note="J domain"                                          
FT   promoter        3531..3876                                                
FT                   /note="SV40"                                              
FT   primer          2735..2757                                                
FT                   /note="mP58.Xho1.4AS"                                     
FT   primer          2780..2804                                                
FT                   /note="SP6"                                               
FT   CDS             3937..4728                                                
FT                   /note="neo                                                
FT                   /codon_start=1"                                           
FT   misc_feature    916..1047                                                 
FT                   /note="Linker + 5'UTR"                                    
FT   primer          863..882                                                  
FT                   /note="T7"                                                
FT   primer          2051..2074                                                
FT                   /note="mP58.3S"                                           
FT   CDS             908..2559                                                 
FT                   /note="fusion ORF                                         
FT                   /codon_start=1"                                           
XX                                                                             
SQ   Sequence   7232 BP;  1793 A; 1802 C; 1860 G; 1777 T;                      
     GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG         
     CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG         
     CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC         
     TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT         
     GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA         
     TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC         
     CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC         
     ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT         
     ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT         
     ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA         
     TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG         
     ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC         
     AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG         
     GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA         
     CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC         
     GAGCTCGGAT CCCCGGAATT CCCGGGATAT CGTCGACCCA CGCGTCCGCG AGCGAGCGAG         
     TGGACTGGGC GCTTCGCTGC GGGCTGTTGG CCGCGCGGGC TGCTGGGCCG CGCACCTTCC         
     TGCTCTTCGG CTCAGGCGCC CTTGGACATG GTGGCCCCCG GCTCGGTGGG CAGCCGGCTG         
     GGCGCGGTGT TCCCGTTCCT GCTGGTCCTG GTGGACCTGC AGTACGAAGG CGCTGAGTGT         
     GGAGTAAATG CGGATGTGGA GAAGCATCTT GAATTGGGGA AGAAATTACT CGCAGCCGGA         
     CAGCTAGCCG ACGCCTTATC ACAGTTTCAC GCTGCAGTTG ATGGTGACCC CGATAACTAT         
     ATCGCATACT ATAGGAGAGC GACCGTCTTC TTAGCCATGG GGAAATCCAA AGCAGCCCTG         
     CCCGACCTCA CCAAAGTGAT TGCGCTGAAG ATGGACTTTA CTGCCGCAAG ACTACAGAGA         
     GGTCACCTAC TGCTCAAACA AGGGAAGCTT GACGAAGCAG AAGACGATTT CAAGAAAGTG         
     CTCAAATCTA ACCCCAGTGA GCAGGAGGAG AAGGAAGCCG AGTCTCAGCT TGTAAAAGCT         
     GACGAGATGC AGCGGCTGCG CTCCCAAGCG CTCGACGCCT TTGACGGTGC CGATTACACT         
     GCTGCTATAA CCTTCCTTGA TAAGATTTTA GAGGTTTGTG TTTGGGATGC AGAATTGCGT         
     GAACTGCGAG CAGAATGCTT CATAAAGGAA GGAGAGCCCA GGAAAGCCAT CAGCGACTTA         
     AAGGCTGCTT CAAAGCTGAA GAGTGACAAC ACAGAGGCGT TTTACAAAAT CAGCACACTC         
     TACTACCAGC TAGGAGACCA TGAGCTGTCT CTCAGTGAAG TTCGTGAATG TCTGAAACTT         
     GACCAGGATC ACAAGCGGTG TTTTGCACAC TATAAGCAAG TGAAGAAACT TAACAAGCTG         
     ATTGAGTCAG CTGAAGAGCT GATCCGAGAT GGCAGATACA CAGATGCAAC CAGCAAATAT         
     GAATCAGTCA TGAAAACAGA GCCCAGTGTT GCTGAGTACA CAGTGCGCTC GAAGGAGAGG         
     ATCTGCCACT GCTTCTCTAA GGATGAGAAG CCCGTGGAAG CCATTAGGAT ATGTTCTGAA         
     GTTTTACAGA TGGAGCCCGA CAATGTGAAT GCTCTGAAAG ACCGAGCCGA GGCCTACTTA         
     ATAGAGGAAA TGTACGATGA AGCCATTCAG GATTATGAAG CTGCTCAGGA GCACAATGAA         
     AATGACCAGC AGATTCGGGA AGGCTTAGAG AAAGCCCAGC GGTTACTGAA GCAGTCACAG         
     AAACGAGATT ATTATAAAAT CTTGGGAGTA AAAAGAAATG CCAAAAAACA AGAAATCATT         
     AAAGCATACC GAAAGTTAGC ACTGCAGTGG CATCCAGATA ATTTCCAGAA TGAAGAAGAA         
     AAGAAAAAAG CAGAGAAAAA GTTCATTGAC ATAGCAGCTG CTAAAGAAGT CCTCTCAGAC         
     CCAGAAATGA GAAAGAAGTT TGATGATGGA GAAGATCCTC TTGATGCAGA GAGTCAACAA         
     GGAGGTGGTG GCAACCCCTT CCACCGAAGT TGGAACTCAT GGCAAGGGTT TAACCCCTTC         
     AGCTCAGGCG GACCGTTCAG ATTTAAGTTC CACTTCAATT AAACCAGCTA TTTTACTGCT         
     CCTCTTCTTT ATTATTATTT TTAACATTAA AAACAAATTT TGTTTGGGAT TCTAATGGAA         
     ACAAAATGGA ACAAATCTTT TAGTTTGTCC ATGACCAAAG AGTTGTTTTA AAGAAAAAAA         
     GCTGAGCTTA TTGTAGACTT AAGGTTGATG GGCCTGTCAA GGTGGGAAGC AAGGAGCTCG         
     AGCATGCATC TAGAGGGCCC TATTCTATAG TGTCACCTAA ATGCTAGAGC TCGCTGATCA         
     GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC         
     TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG         
     CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG         
     GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG         
     GCGGAAAGAA CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA         
     AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG         
     CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA         
     GCTCTAAATC GGGGCATCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC         
     AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT         
     CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA         
     ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC         
     TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG         
     TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC         
     ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA         
     AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC         
     ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT         
     TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA         
     GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC         
     GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC         
     GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA         
     ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT         
     GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG         
     TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA         
     AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT         
     CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG         
     GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG         
     GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC         
     GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT         
     GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC         
     TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT         
     GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT         
     CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC         
     TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA         
     CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA         
     TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG         
     CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT         
     CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC         
     CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT         
     GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG         
     GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT         
     CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT         
     TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC         
     TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG         
     ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG         
     CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC         
     GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG         
     GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT         
     TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG         
     TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT         
     GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC         
     TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT         
     TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC         
     TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA         
     CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT         
     CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC         
     GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT         
     AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC         
     AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG         
     CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG         
     CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC         
     CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA         
     TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG         
     TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT         
     CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA         
     GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG         
     TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA         
     CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT         
     GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA         
     TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT         
     CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT         
     CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA         
     AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT         
     GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC         
     GCACATTTCC CCGAAAAGTG CCACCTGACG TC                                       
//