9E10-tagged mouse PERK K618A mutant in the pro-retroviral vector pBABEpuro

Mammalian expression plasmid encoding kinase dead K618A mutant mouse PERK fused to 9E10 epitope at C-term.

Plasmid sequence in EMBL format:

FH Key Location/Qualifiers
FH
FT CDS 1573..4944
FT /codon_start=1
FT primer 5001..5024
FT /note="pBABE.2AS"
FT misc_feature 920..1309
FT /gene="gag"/note="the start codon of the gag gene
FT has been mutated sothat it does not get
FT expressed. Its presence, however,increases the
FT level of retroviral production"
FT LTR 5957..6582
FT /gene="3'
FT LTR"/product="3' LTR"
FT LTR 1..460
FT /gene="5'
FT LTR"/note="The left LTR serves as the promoter of
FT the subcloned
FT CDS complement(8498..7638)
FT /gene="AmpR"/product="beta lactamase"
FT CDS 5300..5899
FT /gene="Puro-R"/product="puromycin N-acetyltransfe
FT rase"
FT /codon_start=1
FT misc_binding 3424..3426
FT /note="ATP binding"
FT misc_feature 1369..1381
FT /note="LINKER"
FT misc_feature 4911..4945
FT /note="9E10-Xho added"
FT primer 3406..3437
FT /note="PERK2.(K/A).1"
FT primer 4893..4909
FT /note="PERK2.9E10.1"
FT promoter 4953..5295
FT /gene="SV 40 early
FT promoter"/product="SV 40 early promoter"
FT misc_feature 6879..6898
FT /gene="Origin"/product="origin of replcation"
FT primer 1311..1334
FT /note="BABEP.1S"
FT primer 3175..3202
FT /note="PERK2.Sal.1S"
FT sig_peptide 3133..3180
FT /note="hydrophobic patch"
XX
SQ Sequence 8645 BP; 1997 A; 2395 C; 2328 G; 1924 T; 1 Other;
CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGAA
CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
CAAGAACAGA TGGTCCCCAG ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA
TGTTTCCAGG GTGCCCCAAG GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC
AGTTCGCTTC TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC
AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA CCCGTGTATC
CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG CTGTTCCTTG GGAGGGTCTC
CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCATT TGGGGGCTCG TCCGGGATCG
GGAGACCCCT GCCCAGGGAC CACCGACCCA CCACCGGGAG GCAAGCTGGC CAGCAACTTA
TCTGTGTCTG TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA
GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT TCTGAACACC
CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTTGGGGGC CGTTTTTGTG GCCCGACCTG
AGGAAGGGAG TCGATGTGGA ATCCGACCCC GTCAGGATAT GTGGTTCTGG TAGGAGACGA
GAACCTAAAA CAGTTCCCGC CTCCGTCTGA ATTTTTGCTT TCGGTTTGGA ACCGAAGCCG
CGCGTCTTGT CTGCTGCAGC GCTGCAGCAT CGTTCTGTGT TGTCTCTGTC TGACTGTGTT
TCTGTATTTG TCTGAAAATT AGGGCCAGAC TGTTACCACT CCCTTAAGTT TGACCTTAGG
TCACTGGAAA GATGTCGAGC GGATCGCTCA CAACCAGTCG GTAGATGTCA AGAAGAGACG
TTGGGTTACC TTCTGCTCTG CAGAATGGCC AACCTTTAAC GTCGGATGGC CGCGAGACGG
CACCTTTAAC CGAGACCTCA TCACCCAGGT TAAGATCAAG GTCTTTTCAC CTGGCCCGCA
TGGACACCCA GACCAGGTCC CCTACATCGT GACCTGGGAA GCCTTGGCTT TTGACCCCCC
TCCCTGGGTC AAGCCCTTTG TACACCCTAA GCCTCCGCCT CCTCTTCCTC CATCCGCCCC
GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT
CACTCCTTCT CTAGGCGCCG GCCGGATCCC CAGTGTGGTG GTACGTAGAA TTCGGCACGA
GCGATGTCTG CACAAGGCTG TCACTCAGGT GGCAGTGGCT GACACGTGGC CGGGCAGCTC
TGCTGCTGCG GCGCGAAGTC GAGAGGCGGC GGGGTCCGTG GCGCGCGCTC GCATTGCTCC
GAGGCTCCGA GCGGCGATAC GGGCGGGCGC CGACGGCAGG GTCTCCATGC CCGCGCGTGG
GGCGGGCCGC TGATGGAGCG CGCCACCCGG CCCGGGCCGC GCGCGCTGCT GCTGCTTCTG
TTCCTGCTGC TGGGCTGCGC GGCGGGGATC TCTGCGGTCG CGCCCGCCCG CAGTTTGCTT
GCTCCCGCGT CGGAGACAGT GTTTGGCTTA GGGGCAGCGG CCGCCCCGAC TTCGGCCGCG
CGGGTGCCTG CGGTGGCAAC GGCCGAAGTG ACCGTGGAGG ACGCCGAGGC ATTGCCGGCT
GCCGCTGGCG AACCGGAGTC ACGCGCGACG GAGCCCGATG ACGACGTGGA ACTGCGGCCT
CGCGGCAGGT CCTTGGTAAT CATCAGCACT TTAGATGGAC GAATCGCTGC ACTGGATGCC
GAGAATGATG GGAAAAAGCA GTGGGATTTG GACGTGGGGT CTGGTTCCTT GGTTTCATCT
AGCCTCAGCA AGCCAGAGGT GTTTGGGAAC AAGATGATCA TCCCCTCCCT GGATGGAGAC
CTCTTCCAGT GGGACCGGGA CCGAGAGAGC ATGGAGGCCG TCCCCTTCAC GGTGGAGTCC
CTGCTCGAAT CTTCCTACAA GTTTGGAGAT GATGTTGTTC TGGTTGGAGG GAAATCTCTG
ATTACATACG GACTCAGTGC TTACAGTGGA AAGCTGAGGT ATATCTGTTC TGCCTTGGGA
TGTCGCCGAT GGGATAGTGA TGAAATGGAA GAAGAGGAAG ACATCTTGCT TCTGCAGCGT
ACGCAGAAGA CTGTGCGAGC TGTCGGGCCT CGAAGCGGCA GTGAGAAGTG GAATTTCAGT
GTTGGCCACT TTGAACTTCG GTATATTCCA GACATGGAAA CTAGAGCCGG ATTCATTGAA
AGCACCTTTA AACCGGGTGG AAACAAAGAA GACTCTAAAA TTATTTCAGA TGTGGAAGAA
CAAGAAGCCA CCATGCTGGA CACAGTGATA AAAGTTTCCG TTGCTGATTG GAAGGTCATG
GCGTTTAGTA GGAAGGGAGG CCGCCTGGAA TGGGAGTACC AGTTTTGTAC TCCCATCGCG
TCCGCCTGGC TGGTGAGGGA TGGCAAGGTC ATCCCCATCA GCCTGTTTGA TGATACAAGT
TACACAGCCA GCGAAGAAGC CTTGGGAGAC GAAGAAGACA TTGTAGAGGC TGCTCGGGGA
GCCACAGAGA ACAGCGTGTA CTTAGGGATG TACAGAGGCC AGCTGTACCT GCAGTCGTCC
GTCAGGGTCT CAGAAAAGTT CCCTACAAGC CCAAAGGCCT TGGAGTCTGT AAATGGCGAA
AATGCAATTA TTCCTCTGCC GACGATCAAA TGGAAGCCCT TAATCCATTC TCCTTCTAGG
ACTCCTGTCT TGGTTGGGTC TGATGAATTT GACAAATGTC TAAGTAATGA TAAGTATTCC
CACGAAGAAT ACAGTAATGG TGCACTTTCA ATCCTCCAGT ATCCATACGA TAACGGTTAC
TATCTGCCAT ACTACAAGAG AGAAAGGAAT AAGCGGAGCA CGCAGATCAC AGTCAGGTTC
CTGGACAGCC CCCACTACAG CAAGAACATC CGCAAGAAGG ACCCTATCCT CCTGCTGCAC
TGGTGGAAGG AGATATTCGG GACGATCCTG CTTTGCATCG TAGCCACGAC CTTCATCGTG
CGCAGGCTTT TCCATCCTCA GCCCCACAGG CAGCGGAAGG AGTCTGAAAC TCAGTGCCAG
ACTGAAAGTA AATACGACTC CGTGAGTGCC GATGTCAGTG ACAACAGCTG GAATGACATG
AAGTACTCAG GATACGTATC CCGATATCTA ACAGATTTTG AGCCAATTCA GTGCATGGGT
CGTGGTGGCT TTGGCGTTGT CTTTGAAGCT AAAAACAAAG TAGATGACTG CAATTACGCT
ATCGCGAGGA TCCGGCTCCC CAACAGGGAG TTGGCACGGG AGAAGGTAAT GCGGGAAGTT
AAAGCCTTGG CTAAGCTGGA ACACCCAGGC ATTGTGAGGT ATTTCAACGC CTGGCTGGAA
ACCCCACCAG AGAAGTGGCA AGAAGAGATG GATGAGATCT GGCTCAAAGA CGAAAGCACA
GACTGGCCGC TCAGCTCCCC TAGCCCGATG GATGCCCCAT CTGTTAAGAT CCGAAGGATG
GATCCYTTCT CTACAAAAGA GCAGATCGAA GTCATAGCTC CTTCTCCTGA AAGAAGTCGG
TCTTTCTCGG TGGGCATTTC CTGTGGCCAG ACAAGCTCAT CGGAGAGCCA GTTCTCTCCC
CTGGAGTTCT CAGGGACAGA CTGCGGAGAC AACAGTGACT CAGCGGACGC AGCCTACAAC
CTCCAGGACA GTTGCCTGAC GGACTGCGAG GACGTGGAAG ATGGCACCGT GGACGGCAAT
GACGAGGGAC ACTCCTTTGA ACTTTGTCCG TCCGAAGCTT CTCCCTATAC CCGGTCTAGG
GAAGGAACGT CCTCCTCCAT AGTGTTTGAG GACTCTGGCT GCGGCAACGC GTCCAGTAAG
GAGGAGCCCA GAGGGAACCG GCTGCATGAT GGCAACCATT ATGTTAATAA GCTAACTGAT
CTCAAGTGCT CCAGCAGCAG GTCTTCTTCA GAAGCCACCA CCTTGTCTAC CTCCCCTACC
AGGCCAACCA CTCTAAGCTT GGATTTCACC AAGAACACTG TGGGCCAGCT CCAGCCCAGC
TCCCCCAAGG TGTATCTGTA CATTCAGATG CAGCTGTGCA GGAAGGAGAA CCTCAAAGAC
TGGATGAACC GGCGCTGCAG CTTGGAGGAC CGGGAGCACG GCGTGTGCCT GCACATCTTC
CTGCAGATCG CAGAGGCAGT GGAGTTCCTG CACAGCAAGG GACTCATGCA CAGGGACCTC
AAGCCTTCCA ACATATTCTT CACAATGGAT GATGTGGTCA AGGTTGGGGA CTTTGGACTG
GTGACTGCTA TGGACCAAGA TGAAGAAGAG CAGACTGTTC TGACTCCAAT GCCAGCCTAT
GCTACGCACA CGGGACAAGT AGGGACCAAG CTATACATGA GCCCAGAGCA GATTCATGGA
AACAACTACT CCCATAAAGT GGACATCTTC TCTTTAGGCT TGATTCTGTT TGAACTCCTC
TACCCATTCA GCACCCAGAT GGAACGAGTC CGGATTTTAA CTGATGTCAG AAATCTCAAG
TTTCCTCTAC TGTTCACTCA GAAATATCCC CAAGAGCATA TGATGGTTCA AGACATGCTC
TCTCCATCCC CCACGGAGCG GCCTGAAGCC ACAGACATCA TTGAAAATGC CATATTTGAG
AACTTGGAGT TTCCCGGGAA AACGGTTCTG AGACAGCGGT CCCGCTCCAT GAGTTCATCT
GGAACAAAAC ATTCCAGACA GCCCAGCTGC TCGTACACGC CACTGCCTGG CGAGCAGAAG
CTGATCTCTG AGGAGGATCT GTGACTCGAC CCTGTGGAAT GTGTGTCAGT TAGGGTGTGG
AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
CTTTTGCAAA AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG CCACCCGCGA
CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC CCGCCACGCG
CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG AACTCTTCCT
CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG CCGCGGTGGC
GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA TCGGCCCGCG
CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG GCCTCCTGGC
GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT CGCCCGACCA
CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG CCGAGCGCGC
CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT ACGAGCGGCT
CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT GGTGCATGAC
CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG AAAGGAGCGC
ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT
GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG ATGGAACAGC
TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG
AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC
CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA
GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA
ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC GAGCTCAATA
AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG TCGCCCGGGT
ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC GCTGTTCCTT
GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAC ATGCAGCATG
TATCAAAATT AATTTGGTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA TAGTTGCATT
AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT
CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA
AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA
AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC
TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC
CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT
CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT
GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG
AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT
ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA
GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT
GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA
CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
CAAAAAGGAT CTTCACCTAG ATCCTTTTGC GGCCGCAAAT CAATCTAAAG TATATATGAG
TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA
GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGC AGCCTGAATA
TGGGC
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