ID (SL-)5'ATF4.GFP; DNA; 5146 BP.                                         
XX                                                                             
XX                                                                             
DT   30-7-2004                                                               
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
CC   (SL-)5'ATF4.pEGFP-N1 Inserted 402 residues (Residues 94 to 496 of         
CC   5'ATF4-Nae-pEGFP-N2-pBabe) at position 622replacing residues 622          
CC   to 658 Inserted 320 residues (Residues 590 to 910 of RMF.pcDNA3)          
CC   at position 340replacing residues 340 to 613                              
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   CDS             951..1071                                                 
FT                   /note="ATF4 CDS                                           
FT                   /codon_start=1"                                           
FT   primer          525..545                                                  
FT                   /note="CMVP.1S"                                           
FT   5'UTR           670..851                                                  
FT                   /note="ATF4 5'UTR"                                        
FT   CDS             951..1808                                                 
FT                   /note="ATF4-GFP fusion ORF                                
FT                   /codon_start=1"                                           
FT   exon            673..858                                                  
FT                   /note="exon 1 (non-coding)"                               
FT   promoter        1..636                                                    
FT                   /note="CMV promoter"                                      
FT   OOF-ORF         854..1033                                                 
FT                   /note="OOF-ORF2"                                          
FT   CDS             1092..1811                                                
FT                   /note="GFP                                                
FT                   /codon_start=1"                                           
FT   exon            859..1071                                                 
FT                   /note="exon 2"                                            
FT   terminator      1825..2022                                                
FT                   /note="SV40.term"                                         
FT   primer          695..717                                                  
FT                   /note="mATF4.5S"                                          
FT   primer          1220..1239                                                
FT                   /note="EGFP.4AS"                                          
FT   primer          844..868                                                  
FT                   /note="mATF4.m1.3S&4AS"                                   
FT   misc_feature    755..766                                                  
FT                   /note="OOF-ORF"                                           
FT   primer          673..692                                                  
FT                   /note="mATF4.HD3.1S"                                      
FT   rep_origin      852..863                                                  
FT                   /note="f1 ori"                                            
FT   primer          940..968                                                  
FT                   /note="mATF4.BspH.2AS"                                    
FT   CDS             3042..3836                                                
FT                   /note="Neo                                                
FT                   /codon_start=1"                                           
FT   primer          876..898                                                  
FT                   /note="mATF4.7S"                                          
FT   TATA_signal     554..560                                                  
FT                   /note=""                                                  
XX                                                                             
SQ   Sequence   5146 BP;  1225 A; 1406 C; 1360 G; 1155 T;                      
     TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG         
     CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT         
     GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA         
     ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC         
     AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA         
     CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC         
     CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG         
     ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG         
     GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT         
     ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG         
     GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GGGCCCCCCC         
     TCGAGCTCAA GCTTTTCTGC TTGCTGTCTG CCGGTTTGAG TTGTGCGCTC GGGTGTCCCT         
     TTCCTCTTCC CCTCCCGCAG GGCTTGCGGC CACCATGGCG TATTAGAGGC AGCAGTGCCT         
     GCGGCAGCGT TGGCCTTTGC AGCGGCGGCA GCAGCACCAG GCTCTGCAGC GGCAACCCCC         
     ACCGGCCTAA GCCATGGCGC TCTTCACGAA ATCCAGCAGC AGTGTTGCTG TAACGGACAA         
     AGATACCTTC GAGTTAAGCA CATTCCTGGA ATCCAGCAAA GCCCCACAAC ATGACCGAGA         
     TGAGCTTCCT GAACAGCGAA GTGTTGGCGG GGGACTTGAT GTCCCCCTTC GACCAGTCGG         
     GTTTGGGGGC TGAAGAAAGC CTAGGTCTCT TAGATGACTA TCTGGAGGTG GGGGATCCAC         
     CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG         
     TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG         
     ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC         
     CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG         
     ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC         
     GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG         
     GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA         
     TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA         
     AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG         
     TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC         
     CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG         
     ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC         
     TGTACAAGTA AAGCGGCCGC GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT         
     TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC         
     AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT         
     CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT         
     CATCAATGTA TCTTAAGGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA         
     ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA         
     AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC         
     TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC         
     CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA         
     ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG         
     CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG         
     TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG         
     GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT         
     CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA         
     GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA         
     GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC         
     CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA         
     TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG         
     TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC         
     CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT         
     TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT         
     TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC         
     AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC         
     TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC         
     TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG         
     CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC         
     TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG         
     ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC         
     GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC         
     CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA         
     CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA         
     TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG         
     ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG         
     CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG         
     GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA         
     TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG         
     GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT         
     AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG         
     ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG         
     CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC         
     CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG         
     GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT         
     TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT         
     CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC         
     TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT         
     ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG         
     CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA         
     CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC         
     TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA         
     TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC         
     GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA         
     AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG         
     GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG         
     ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG         
     CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC         
     TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC ATGCAT                        
//