ID   5'3'ATF4.GFP; DNA; 5275 BP.                                         
XX                                                                             
XX                                                                             
DT   19-JAN-1998                                                               
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
CC   5'3'ATF4.pEGFP-N1C2.V1 Inserted 402 residues (Residues 94 to 496          
CC   of 5'ATF4-Nae-pEGFP-N2-pBabe) at position 622replacing residues           
CC   622 to 658 Inserted 1149 residues (Residues 600 to 1749 of                
CC   pEGFP-C2.mATF4.term.V1) at position 1032replacing residues 1032 to        
CC   2005                                                                      
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   CDS             3171..3965                                                
FT                   /note="Neo                                                
FT                   /codon_start=1"                                           
FT   promoter        1..589                                                    
FT                   /note="CMV promoter"                                      
FT   TATA_signal     554..560                                                  
FT                   /note=""                                                  
FT   rep_origin      2191..2646                                                
FT                   /note="F1 Ori"                                            
FT   misc_feature    708..719                                                  
FT                   /note="OOF-ORF"                                           
FT   CDS             904..1024                                                 
FT                   /note="ATF4 CDS                                           
FT                   /codon_start=1"                                           
FT   primer          829..851                                                  
FT                   /note="mATF4.7S"                                          
FT   primer          1776..1799                                                
FT                   /note="mATF4.BamH1.9S"                                    
FT   CDS             904..1842                                                 
FT                   /note="ATF4-GFP fusion ORF                                
FT                   /codon_start=1"                                           
FT   primer          806..821                                                  
FT                   /note="mATF4.m1.3S&4AS"                                   
FT   primer          1173..1192                                                
FT                   /note="GFP.4AS"                                           
FT   primer          648..670                                                  
FT                   /note="mATF4.5S"                                          
FT   primer          626..645                                                  
FT                   /note="mATF4.HD3.1S"                                      
FT   5'UTR           623..804                                                  
FT                   /note="ATF4 5'UTR"                                        
FT   primer          893..921                                                  
FT                   /note="mATF4.BspH.2AS"                                    
FT   rep_origin      805..816                                                  
FT                   /note="f1 ori"                                            
FT   exon            626..811                                                  
FT                   /note="exon 1 (non-coding)"                               
FT   exon            812..1024                                                 
FT                   /note="exon 2"                                            
FT   misc_feature    1923..2186                                                
FT                   /note="mATF4 genomic 3' flank"                            
FT   exon            1776..1922                                                
FT                   /note="exon 3 ATF4"                                       
FT   polyA_signal    1900..1904                                                
FT                   /note="putative poly-A"                                   
FT   primer          1349..1368                                                
FT                   /note="EGFP.4AS"                                          
FT   primer          797..821                                                  
FT                   /note="mATF4.m1.3S&4AS"                                   
FT   OOF-ORF         807..986                                                  
FT                   /note="OOF-ORF2"                                          
XX                                                                             
SQ   Sequence   5275 BP;  1238 A; 1412 C; 1436 G; 1189 T;                      
     TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG         
     CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT         
     GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA         
     ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC         
     AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA         
     CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC         
     CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG         
     ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG         
     GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT         
     ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA         
     CCGGACTCAG ATCTCGAGCT CAAGCTTTTC TGCTTGCTGT CTGCCGGTTT GAGTTGTGCG         
     CTCGGGTGTC CCTTTCCTCT TCCCCTCCCG CAGGGCTTGC GGCCACCATG GCGTATTAGA         
     GGCAGCAGTG CCTGCGGCAG CGTTGGCCTT TGCAGCGGCG GCAGCAGCAC CAGGCTCTGC         
     AGCGGCAACC CCCACCGGCC TAAGCCATGG CGCTCTTCAC GAAATCCAGC AGCAGTGTTG         
     CTGTAACGGA CAAAGATACC TTCGAGTTAA GCACATTCCT GGAATCCAGC AAAGCCCCAC         
     AACATGACCG AGATGAGCTT CCTGAACAGC GAAGTGTTGG CGGGGGACTT GATGTCCCCC         
     TTCGACCAGT CGGGTTTGGG GGCTGAAGAA AGCCTAGGTC TCTTAGATGA CTATCTGGAG         
     GTGGGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG         
     GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC         
     GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC         
     AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC         
     AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC         
     TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG         
     GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG         
     GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT         
     ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC         
     GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC         
     CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC         
     AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC         
     GGCATGGACG AGCTGTACAA GTCCGGCCGG ACTCAGATCC AGTATCTGAA AGACCTGATA         
     GAAGAGGTCC GTAAGGCAAG GGGGAAGAAG AGAGTTCCGT AATAGGGTAG TCAGGTGCTT         
     TGTGCTTGTA CATAGTCTTG TGTTGCTGTG TTTGCTGTAA TAAATTATTT TGTAGTGAAA         
     GTACCTATGT GGGTTTTCAG TGTCATTCTT TGTAATAGAC TTAACTGCAG ACGTTCCTGG         
     GTTAGATACA ATAACTACCT GGGTATATCC CAGGGCTGAG CTGAGCTGTT TGAGACGGTG         
     AAGATGAGGG AGCTCTGGGT TAATGAAAGG AACAGCCTGA ATTCCACCTG TTCATACTGG         
     CTTTGTGCCA GATAGCTGCC ATGTAAGTAC CTAGATGAGG TACCCGCCTT GAGCACACAG         
     AGGATTAGGT TTTTCTCAGG CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT         
     TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA         
     TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA         
     AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG         
     GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA         
     AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG         
     CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA         
     GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG         
     GCGCGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT         
     AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT         
     ATTGAAAAAG GAAGAGTCCT GAGGCGGAAA GAACCAGCTG TGGAATGTGT GTCAGTTAGG         
     GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA         
     GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT         
     GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC         
     TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA         
     GGCCGAGGCC GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG         
     CCTAGGCTTT TGCAAAGATC GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC         
     AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT         
     GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC         
     GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG         
     CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG         
     TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT         
     CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC         
     ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG         
     CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG         
     GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC         
     TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT         
     CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG         
     CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT         
     ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT         
     TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG         
     AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA         
     CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCTAG         
     GGGGAGGCTA ACTGAAACAC GGAAGGAGAC AATACCGGAA GGAACCCGCG CTATGACGGC         
     AATAAAAAGA CAGAATAAAA CGCACGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG         
     GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG         
     CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC         
     CAACGTCGGG GCGGCAGGCC CTGCCATAGC CTCAGGTTAC TCATATATAC TTTAGATTGA         
     TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT         
     GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT         
     CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA         
     ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA         
     GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT         
     AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT         
     ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA         
     GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT         
     GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC         
     GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA         
     GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG         
     CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA         
     AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT         
     GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCA TGCAT              
//