ID   5'ATF4.uORF2.AUA.GFP; DNA; 5099 BP.                                         
XX                                                                             
XX                                                                             
DT   30-JUL-2004                                                               
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
XX                                                                             
CC   5'ATF4.muORF2ATA.pEGFP-N1 Inserted 402 residues (Residues 94 to           
CC   496 of 5'ATF4-Nae-pEGFP-N2-pBabe) at position 622replacing                
CC   residues 622 to 658 Typed -1 residues at position 808 Typed 1             
CC   residues at position 808                                                  
XX                                                                             
XX                                                                             
FH   Key             Location/Qualifiers                                       
FH                                                                             
FT   TATA_signal     554..560                                                  
FT                   /note=""                                                  
FT   terminator      1778..1975                                                
FT                   /note="SV40.term"                                         
FT   promoter        1..589                                                    
FT                   /note="CMV promoter"                                      
FT   CDS             904..1761                                                 
FT                   /note="ATF4-GFP fusion ORF                                
FT                   /codon_start=1"                                           
FT   CDS             2995..3789                                                
FT                   /note="Neo                                                
FT                   /codon_start=1"                                           
FT   5'UTR           623..804                                                  
FT                   /note="ATF4 5'UTR"                                        
FT   mutation        807..809                                                  
FT                   /note="ATG-->ATA mutation in uORF2"                       
FT   OOF-ORF         807..986                                                  
FT                   /note="OOF-ORF2"                                          
FT   primer          1173..1192                                                
FT                   /note="EGFP.4AS"                                          
FT   primer          829..851                                                  
FT                   /note="mATF4.7S"                                          
FT   CDS             1045..1764                                                
FT                   /note="GFP                                                
FT                   /codon_start=1"                                           
FT   primer          893..921                                                  
FT                   /note="mATF4.BspH.2AS"                                    
FT   rep_origin      805..816                                                  
FT                   /note="f1 ori"                                            
FT   primer          648..670                                                  
FT                   /note="mATF4.5S"                                          
FT   primer          354..373                                                  
FT                   /note="pEGFP.N1-1S"                                       
FT   primer          626..645                                                  
FT                   /note="mATF4.HD3.1S"                                      
FT   primer          806..821                                                  
FT                   /note="mATF4.m1.3S&4AS"                                   
FT   primer          797..821                                                  
FT                   /note="mATF4.m1.3S&4AS"                                   
FT   exon            626..811                                                  
FT                   /note="exon 1 (non-coding)"                               
FT   CDS             904..1024                                                 
FT                   /note="ATF4 CDS                                           
FT                   /codon_start=1"                                           
FT   exon            812..1024                                                 
FT                   /note="exon 2"                                            
FT   misc_feature    708..719                                                  
FT                   /note="OOF-ORF"                                           
XX                                                                             
SQ   Sequence   5099 BP;  1212 A; 1390 C; 1352 G; 1145 T;                      
     TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG         
     CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT         
     GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA         
     ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC         
     AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA         
     CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC         
     CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG         
     ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG         
     GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT         
     ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA         
     CCGGACTCAG ATCTCGAGCT CAAGCTTTTC TGCTTGCTGT CTGCCGGTTT GAGTTGTGCG         
     CTCGGGTGTC CCTTTCCTCT TCCCCTCCCG CAGGGCTTGC GGCCACCATG GCGTATTAGA         
     GGCAGCAGTG CCTGCGGCAG CGTTGGCCTT TGCAGCGGCG GCAGCAGCAC CAGGCTCTGC         
     AGCGGCAACC CCCACCGGCC TAAGCCATAG CGCTCTTCAC GAAATCCAGC AGCAGTGTTG         
     CTGTAACGGA CAAAGATACC TTCGAGTTAA GCACATTCCT GGAATCCAGC AAAGCCCCAC         
     AACATGACCG AGATGAGCTT CCTGAACAGC GAAGTGTTGG CGGGGGACTT GATGTCCCCC         
     TTCGACCAGT CGGGTTTGGG GGCTGAAGAA AGCCTAGGTC TCTTAGATGA CTATCTGGAG         
     GTGGGGGATC CACCGGTCGC CACCATGGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG         
     GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC         
     GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC         
     AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC         
     AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC         
     TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG         
     GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG         
     GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT         
     ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC         
     GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC         
     CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCGCCCTGAG CAAAGACCCC         
     AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC         
     GGCATGGACG AGCTGTACAA GTAAAGCGGC CGCGACTCTA GATCATAATC AGCCATACCA         
     CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA CCTCCCCCTG AACCTGAAAC         
     ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT         
     AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG         
     GTTTGTCCAA ACTCATCAAT GTATCTTAAG GCGTAAATTG TAAGCGTTAA TATTTTGTTA         
     AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC         
     AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG         
     AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT         
     CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC         
     CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG         
     CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG         
     GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA         
     CAGGGCGCGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT         
     TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA         
     TAATATTGAA AAAGGAAGAG TCCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT         
     TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA         
     ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA         
     GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC         
     TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG         
     CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG         
     GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT         
     GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT         
     GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG         
     GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAAGAC         
     GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC         
     GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC         
     CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG         
     CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG         
     CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT         
     CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC CGACGGCGAG         
     GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC         
     TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG         
     TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG         
     CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG         
     TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT         
     CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC         
     GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC         
     CTAGGGGGAG GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA         
     CGGCAATAAA AAGACAGAAT AAAACGCACG GTGTTGGGTC GTTTGTTCAT AAACGCGGGG         
     TTCGGTCCCA GGGCTGGCAC TCTGTCGATA CCCCACCGAG ACCCCATTGG GGCCAATACG         
     CCCGCGTTTC TTCCTTTTCC CCACCCCACC CCCCAAGTTC GGGTGAAGGC CCAGGGCTCG         
     CAGCCAACGT CGGGGCGGCA GGCCCTGCCA TAGCCTCAGG TTACTCATAT ATACTTTAGA         
     TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC         
     TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA         
     AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA         
     AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC         
     CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT         
     AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC         
     TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC         
     GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA         
     GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG         
     CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG         
     GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT         
     TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT         
     GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC         
     ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCATGCAT          
//