ID human eIF2a WT in pcDNA3.CD2; circular DNA; 7421 BP. XX XX DT XX XX XX XX XX CC Inserted 5432 residues at position 0 Inserted 5446 residues at CC position 0replacing residues 0 to 5432 Inserted 1328 residues CC (Residues 2078 to 3406 of pCDNA3.hCD2) at position 2092replacing CC residues 2092 to 3064 Deleted residues 2092 to 2116 Inserted 54 CC residues (Residues 652 to 706 of Complement of pcDna3.cd2/2AS) at CC position 2092 Typed 2 residues at position 943 Inserted 1415 CC residues (Residues 3013 to 4428 of Copy of pcDNA1.1amp) at CC position 938replacing residues 938 to 985 Typed 2 residues at CC position 2340 Inserted 84 residues (Residues 0 to 84 of CC Untitled-2) at position 937replacing residues 937 to 972 Inserted CC 435 residues (Residues 242 to 677 of IN@T7c) at position CC 2174replacing residues 2174 to 2404 Deleted residues 0 to 938 CC Inserted 938 residues (Residues 0 to 938 of pCDNA3-CD2) at CC position 0 Deleted residues 2609 to 7458 Inserted 4849 residues CC (Residues 983 to 5832 of pCDNA3-CD2) at position 2609 Deleted CC residues 889 to 938 Inserted 12 residues (Residues 38 to 50 of CC heIF2a.pBS(1999)) at position 889 XX XX FH Key Location/Qualifiers FH FT CDS 985..1932 FT /note="eif2alpha FT /codon_start=1" FT CDS 3736..4788 FT /note="hCD2 FT /codon_start=1" FT misc_feature 1138 FT /note="serine residue phosphorylated by eif2A FT kinases (amino acid residue 52 in the human~51 FT in the yeast)" FT promoter 3334..3679 FT /note="SV40" FT primer 3848..3867 FT /note="hCD2.2AS (SEQ)" FT primer 4756..4775 FT /note="hCD2.1S (SEQ)" FT misc_feature 209..888 FT /note="cheched by sequencing" FT unsure 1933..2565 FT /note="" FT primer 2583..2607 FT /note="SP6" FT misc_feature 4790..5559 FT /note="cheched by sequencing" FT primer 863..882 FT /note="T7" XX SQ Sequence 7421 BP; 2002 A; 1755 C; 1736 G; 1928 T; GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGATATC GAATTCCGGG GAAGCAAGTC TGGTCTCTGT GATTGAAGAA GTCGGCTCTG GGCTCCAGTG CGGGAATCAC ACACATACCT CAGAATGCCG GGTCTAAGTT GTAGATTTTA TCAACACAAA TTTCCTGAGG TGGAAGATGT AGTGATGGTG AATGTCAGAT CCATTGCTGA AATGGGGGCT TATGTCAGCT TGCTGGAATA CAACAACATT GAAGGCATGA TTCTTCTTAG TGAATTATCC AGAAGGCGTA TCCGTTCTAT CAACAAACTC ATCCGAATTG GCAGGAATGA GTGTGTGGTT GTCATTAGGG TGGACAAAGA AAAAGGATAT ATTGATTTGT CAAAAAGAAG AGTTTCTCCA GAGGAAGCAA TCAAATGTGA AGACAAATTC ACAAAATCCA AAACTGTTTA TAGCATTCTT CGTCATGTTG CTGAGGTGTT AGAATACACC AAGGATGAGC AGCTGGAAAG CCTATTCCAG AGGACTGCCT GGGTCTTTGA TGACAAGTAC AAGAGACCTG GATATGGTGC CTATGATGCA TTTAAGCATG CAGTCTCAGA CCCATCTATT TTGGATAGTT TAGATTTGAA TGAAGATGAA CGGGAAGTAC TCATTAATAA TATTAATAGG CGCTTGACCC CACAGGCTGT CAAAATTCGA GCAGATATTG AAGTGGCTTG TTATGGTTAT GAAGGCATTG ATGCTGTAAA AGAAGCCCTA AGAGCAGGTT TGAATTGTTC TACAGAAAAC ATGCCCATTA AGATTAATCT AATAGCTCCT CCTCGGTATG TAATGACTAC GACAACCCTG GAGAGAACAG AAGGCCTTTC TGTCCTCAGT CAAGCTATGG CTGTTATCAA AGAGAAGATT GAGGAAAAGA GGGGTGTGTT CAATGTTCAA ATGGAGCCCA AAGTGGTCAC AGATACAGAT GAGACTGAAC TTGCGAGGCA GATGGAGAGG CTTGAAAGAG AAAATGCCGA AGTGGATGGA GATGATGATG CAGAAGAAAT GGAAGCCAAA GCTGAAGATT AACTTTGTGG GAAACAGAGT CCAATTTAAG GAACACAGAG CAGCGCTTCC TGGCTGTAAA TCCTAGACTT GAAAGTTTTC CAGTATTGAA AACTTCAAAG CTGAATATTT TTTATTTCTA AGTATTTAAA TGTTCTAACA GATCAGAACA TGAAATGCCC TCCTAAATGT CAGCTGTTGT CACACAGTAG CTCCAACACT TTGAGCATTT TTAAGGGAGT GGCCTCATTT CACTAGAGAC AAATCTTTAA GAATAGTTCT AAAATTGGGC TTGTGATTTC CATTTCTGAT GTCTCCAGAT TGGCACCCCT TTCTAGTTCA ATGCCTCACG AGATTTGCCA GGGGCATCCA AGGCAAACAA TCCCAATCTT TCTATATAAA ATGTATTCAA GCAAACATCA AATAAATTTC TGGGATATTT AACTATAGGC TTCTTCCTTC TTGTCACCAG TTAAAAGCAT TTTAATACTA AGACCCTAAT TCTTTTATCT TTATTTTAGT CTTGATGTGG AACTGTAGGA GCAGGTGAAT AAAGGATCTC TATAACAGAT CCTTTCAAAA GAAGAGTTTT AGAGAAAATA AATTTAACTT TAACCACAGT GAAAGTTGAC CTCGAGCATG CAGCCCGGGG GATCCACTAG TTCTAGAGGG CCCTATTCTA TAGTGTCACC TAAATGCTAG AGCTCGCTGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGCTCGAC TAGAGATCCC TCGACCTCGA GATCCATTGT GCTCTAAAGC CTAAGATGAG CTTTCCATGT AAATTTGTAG CCAGCTTCCT TCTGATTTTC AATGTTTCTT CCAAAGGTGC AGTCTCCAAA GAGATTACGA ATGCCTTGGA AACCTGGGGT GCCTTGGGTC AGGACATCAA CTTGGACATT CCTAGTTTTC AAATGAGTGA TGATATTGAC GATATAAAAT GGGAAAAAAC TTCAGACAAG AAAAAGATTG CACAATTCAG AAAAGAGAAA GAGACTTTCA AGGAAAAAGA TACATATAAG CTATTTAAAA ATGGAACTCT GAAAATTAAG CATCTGAAGA CCGATGATCA GGATATCTAC AAGGTATCAA TATATGATAC AAAAGGAAAA AATGTGTTGG AAAAAATATT TGATTTGAAG ATTCAAGAGA GGGTCTCAAA ACCAAAGATC TCCTGGACTT GTATCAACAC AACCCTGACC TGTGAGGTAA TGAATGGAAC TGACCCCGAA TTAAACCTGT ATCAAGATGG GAAACATCTA AAACTTTCTC AGAGGGTCAT CACACACAAG TGGACCACCA GCCTGAGTGC AAAATTCAAG TGCACAGCAG GGAACAAAGT CAGCAAGGAA TCCAGTGTCG AGCCTGTCAG CTGTCCAGAG AAAGGTCTGG ACATCTATCT CATCATTGGC ATATGTGGAG GAGGCAGCCT CTTGATGGTC TTTGTGGCAC TGCTCGTTTT CTATATCACC AAAAGGAAAA AACAGAGGAG TCGGAGAAAT GATGAGGAGC TGGAGACAAG AGCCCACAGA GTAGCTACTG AAGAAAGGGG CCGGAAGCCC CACCAAATTC CAGCTTCAAC CCCTCAGAAT CCAGCAACTT CCCAACATCC TCCTCCACCA CCTGGTCATC GTTCCCAGGC ACCTAGTCAT CGTCCCCCGC CTCCTGGACA CCGTGTTCAG CACCAGCCTC AGAAGAGGCC TCCTGCTCCG TCGGGCACAC AAGTTCACCA GCAGAAAGGC CCGCCCCTCC CCAGACCTCG AGTTCAGCCA AAACCTCCCC ATGGGGCAGC AGAAAACTCA TTGTCCCCTT CCTCTAATTA AAAAAGATAG AAACTGTATT TTTCAATAAA AAGCACTGTG GATTTCTGCC CTCCTGATGT GCATATCCGT ACTTCCATGA GGTGTTTTCT GTGTGCAGAA CATTGTCACC TCCTGAGGCT GTGGGCCACA GCCACCTCTG CATCTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT C //