pCDNA3-hCD2

pCDNA3 Mammalian expression vecin which the NeoR selection marker has been replaced by the coding region of human CD2, a surface protein that is recognizable by various commercially-available fluorescent conjugated antibodies

ID   pCDNA3-CD2; 5832 BP.                               

XX                                                                            

XX                                                                            

DT   2000                                                              

XX                                                                            

CC    Inserted hCD2 cDNA, replacing the Neo resistance cassette of pCDNA3          

XX                                                                            

XX                                                                            

FH   Key             Location/Qualifiers                                      

FH                                                                            

FT   primer          2259..2278                                               

FT                   /note="hCD2.2AS"                                         

FT   promoter        209..838                                                 

FT                   /note="CMV promoter"                                     

FT   promoter        1745..2090                                               

FT                   /note="SV40"                                             

FT   primer          994..1018                                                

FT                   /note="SP6"                                              

FT   CDS             2147..3199                                               

FT                   /note="hCD2                                               

FT                   /codon_start=1"                                          

FT   primer          3167..3186                                               

FT                   /note="hCD2.1S"                                          

FT   primer          863..882                                                 

FT                   /note="T7"                                               

XX                                                                            

SQ   Sequence   5832 BP;  1487 A; 1485 C; 1384 G; 1476 T;                     

     GACGGATCGG GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG        

     CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG        

     CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC        

     TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT        

     GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA        

     TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC        

     CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC        

     ATTGACGTCA ATGGGTGGAC TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT        

     ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT        

     ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA        

     TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG        

     ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC        

     AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG        

     GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA        

     CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC        

     GAGCTCGGAT CCACTAGTAA CGGCCGCCAG TGTGCTGGAA TTCTGCAGAT ATCCATCACA        

     CTGGCGGCCG CTCGAGCATG CATCTAGAGG GCCCTATTCT ATAGTGTCAC CTAAATGCTA        

     GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT        

     CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG        

     AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC        

     AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT        

     CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT        

     GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG        

     CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG        

     GCTTTCCCCG TCAAGCTCTA AATCGGGGCA TCCCTTTAGG GTTCCGATTT AGTGCTTTAC        

     GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT        

     GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT        

     TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT        

     TGGGGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT        

     AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGGCAGGCA        

     GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT         

     CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC        

     CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG        

     GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC        

     AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGCTCGA        

     CTAGAGATCC CTCGACCTCG AGATCCATTG TGCTCTAAAG CCTAAGATGA GCTTTCCATG        

     TAAATTTGTA GCCAGCTTCC TTCTGATTTT CAATGTTTCT TCCAAAGGTG CAGTCTCCAA        

     AGAGATTACG AATGCCTTGG AAACCTGGGG TGCCTTGGGT CAGGACATCA ACTTGGACAT        

     TCCTAGTTTT CAAATGAGTG ATGATATTGA CGATATAAAA TGGGAAAAAA CTTCAGACAA        

     GAAAAAGATT GCACAATTCA GAAAAGAGAA AGAGACTTTC AAGGAAAAAG ATACATATAA        

     GCTATTTAAA AATGGAACTC TGAAAATTAA GCATCTGAAG ACCGATGATC AGGATATCTA        

     CAAGGTATCA ATATATGATA CAAAAGGAAA AAATGTGTTG GAAAAAATAT TTGATTTGAA        

     GATTCAAGAG AGGGTCTCAA AACCAAAGAT CTCCTGGACT TGTATCAACA CAACCCTGAC        

     CTGTGAGGTA ATGAATGGAA CTGACCCCGA ATTAAACCTG TATCAAGATG GGAAACATCT        

     AAAACTTTCT CAGAGGGTCA TCACACACAA GTGGACCACC AGCCTGAGTG CAAAATTCAA        

     GTGCACAGCA GGGAACAAAG TCAGCAAGGA ATCCAGTGTC GAGCCTGTCA GCTGTCCAGA        

     GAAAGGTCTG GACATCTATC TCATCATTGG CATATGTGGA GGAGGCAGCC TCTTGATGGT        

     CTTTGTGGCA CTGCTCGTTT TCTATATCAC CAAAAGGAAA AAACAGAGGA GTCGGAGAAA        

     TGATGAGGAG CTGGAGACAA GAGCCCACAG AGTAGCTACT GAAGAAAGGG GCCGGAAGCC        

     CCACCAAATT CCAGCTTCAA CCCCTCAGAA TCCAGCAACT TCCCAACATC CTCCTCCACC        

     ACCTGGTCAT CGTTCCCAGG CACCTAGTCA TCGTCCCCCG CCTCCTGGAC ACCGTGTTCA        

     GCACCAGCCT CAGAAGAGGC CTCCTGCTCC GTCGGGCACA CAAGTTCACC AGCAGAAAGG        

     CCCGCCCCTC CCCAGACCTC GAGTTCAGCC AAAACCTCCC CATGGGGCAG CAGAAAACTC        

     ATTGTCCCCT TCCTCTAATT AAAAAAGATA GAAACTGTAT TTTTCAATAA AAAGCACTGT        

     GGATTTCTGC CCTCCTGATG TGCATATCCG TACTTCCATG AGGTGTTTTC TGTGTGCAGA        

     ACATTGTCAC CTCCTGAGGC TGTGGGCCAC AGCCACCTCT GCATCTTCGA AATGACCGAC        

     CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG        

     TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC        

     ATGCTGGAGT TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA        

     AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT        

     TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC        

     TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA        

     CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA         

     CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG        

     CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC        

     GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT        

     CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG        

     TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC        

     CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA        

     AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT        

     CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG        

     GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG        

     CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT        

     CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC        

     AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC        

     TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC        

     GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT        

     TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC        

     TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG        

     AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA        

     ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA        

     CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG        

     ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC        

     CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC        

     AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT        

     AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC        

     GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG        

     CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC        

     GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT        

     TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG        

     TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT        

     AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG        

     CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA        

     CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA        

     AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC         

     TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA        

     TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG        

     CCACCTGACG TC                                                            

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