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ID pFLAG.CReP(24-698).CMV2
DNA; 6690 BP.
XX
XX
DT February
3, 2004
XX
XX
XX
XX
XX
CC This is a predicted sequence assembled
from sequenced fragments. CC The
plasmid itself was not fully sequenced. XX
XX
FH
Key
Location/Qualifiers
FH
FT
promoter 308..892
FT
/note="CMV promoter"
FT
CDS
925..2973
FT
/note="FLAG-G34L(24-698)
FT
/codon_start=1"
FT
misc_feature
928..951
FT
/note="FLAG epitope"
FT
misc_feature
2749..2766
FT
/note="PP1-binding"
FT
polyA_signal
3028..3654
FT
/note="hGH PolyA"
XX
SQ
Sequence 6690
BP; 1631 A; 1720 C; 1655 G; 1684
T;
CCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC
TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCCA
GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTGAT CTAATCAATA
TTGGCCATTA GCCATATTAT TCATTGGTTA TATAGCATAA ATCAATATTG GCTATTGGCC
ATTGCATACG TTGTATCCAT ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT
ACCGCCATGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT
AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
CGACCGCCCA GCGACCCCCG CCCGTTGACG TCAATAGTGA CGTATGTTCC CATAGTAACG
CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
GCAGTACATC AAGTGTATCA TATGCCAAGT CCGCCCCCTA TTGACGTCAA TGACGGTAAA
TGGCCCGCCT AGCATTATGC CCAGTACATG ACCTTACGGG AGTTTCCTAC TTGGCAGTAC
ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CACCAATGGG
CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAATAA CCCCGCCCCG
TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
GTGAACCGTC AGAATTGATC TACCATGGAC TACAAAGACG ATGACGACAA GCTTCGGCGA
TCGCACGCCT GCTCTTCGGA GTTCCCGCCG CCTTCCTCTC GACAAAATCC CGGGAACTCC
GCTCTGCCCG AGCGTCGGAC CAGGTACTGG ACCAAATTGC TTTCTCAGCT CCTTGCCCTG
CTCCCTAGCC TATTCCAGAA GCTGCTGCTT TGGAGCCAGC TTTCCGGGGG CCTGATTCCT
ACCAGATGGC TAGATTTTGC CGCAAGTTAC AGCGCCCTGA GAGCTTCGAG AGGACGGGAG
GAATCTGACG CTCCCACGGT GCAGAAGTCT CTGAGTTACA CTGCGGCTGG ACTCTTCGCG
AAGACTCGCG TCGTCAGTAC TCTTGCATTG GCTAGAGGAG GGACTCCAGT GGCAGTGCTC
GTCCTCAGAC TGGAAGTTAA ACTCAAGGCC CAGGAAAGAG CTTTAGACTC TGCAGCGCCC
ACTTTCCTCC TGGAGCAGCA GCTGTGGGGA GTGGAGTTGC TGCCCAGTAG CCTTCAAGCT
GGTCTAGTCT CCCACCGAGA ACTTGACTCT TCATCCTCTG GGCCTCTGAG CGTTCAGAGC
TTAGGTAATT TCAAGGTAGT TTCCTATCTC CTGAACCCTT CCTACCTGGA CTACCTTCCC
CAGTTAGGGC TGCGCTGTCA GAGCAGCGCT GGAGGTGGCC AGTTTGTGGG TTTCCGAACA
CTAACCCCAG AGAGCTGCTA TCTTTCTGAA GATGGTTGTC ACCCTCAGCC GTTGCGGGCA
GAGATGTCGG CAACCGCCTG GAGAAGGTGT CCGCCTCTCT CTACAGAAGG CCTGCCGGAA
ATCCACCACC GTAGAATGAG ATGGCTAGTA TTCCTACAGC CTAACCAAGG CCAAGACTTA
CCCACCCTTG ACCAAGATAA TGGCTATCAT AGCTTGGAGG AGGAACATAA CCTTCTCCGG
ATGGACCCAC AACATTGCAC AGATAACCCA GCACAGGCGG TGTCCCCTGC TGCAGACAGG
CCGGAGCCCA CTGAGAAAAA ACCAGAATTG GTGATTCAAG AAGTTTCACA GAGCCCCCAG
GGAAGCAGTC TGTTTTGTGA ATTACCCGTG GAAAAAGAAT GTGAAGAGGA CCACACTAAT
GCAACTGACC TCTCAGATAG AGGAGAGAGC CTTCCTGTTT CTACCAGACC AGTTTGTAGC
AACAAACTGA TAGATTATAT TTTGGGAGGC GCCCCCAGTG ACTTGGAAGC CAGCTCTGAT
TCTGAAAGTG AGGATTGGGG CGAGGAACCT GAGGACGATG GCTTTGATAG CGATGGCTCC
CTGTCTGAAT CAGACGTGGA ACAGGACTCG GAAGGCCTTC ACCTTTGGAA CTCTTTCCAC
AGTGTAGATC CTTACAAACC CCAAAACTTT ACAGCCACGA TTCAGACGGC TGCCAGAATT
GCCCCCAGAG ACCCATCAGA TTCAGGGACA TCCTGGTCTG GCAGCTGTGG TGTAGGGAGC
TGTCAGGAGG GACCCCTTCC GGAGACCCCC GACCATAGTT CCGGGGAGGA AGATGACTGG
GAACCGAGTG CAGATGAAGC AGAGAATCTT AAATTGTGGA ACTCTTTCTG TCATTCTGAG
GACCCCTACA ACCTTTTAAA TTTTAAGGCT CCTTTTCAAC CGTCAGGGAA GAATTGGAAA
GGCCGTCAGG ACTCAAGAGG CTCTTCTGAG GTCACAGTGG CCTTCTCTGG CCATCATACC
TTACTTTCTT GTAAGGCCCA GCTGTTAGAG AGCCAAGAAG ATAATTGTCC AGGCTGTGGG
CTGGGTGAGG CTCTTGCTGG AGAAAGATAC ACCCATATCA AGAGAAAAAA GGTAACCTTC
CTGGAAGAAG TTACTGAGTA TTATATAAGT GGTGATGAGG ATCGCAAAGG ACCATGGGAA
GAATTTGCAA GGGATGGATG CAGGTTCCAG AAACGAATTC AAGAAACAGA AGTTGCCATT
GGCTACTGCT TGGCCTTTGA GCACAGAGAA AAAATGTTTA ATAGACTGAG GATCGAGTCA
AAGGACTTAC TGTTGTACAG CAATGTTAAG AAGTGAACAG CCTGCAACCC GTGCCCACTC
TGTCTCTTAC TCGACTCTAG AGGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC
TCTCCTGGCC TTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG
TTGCATCATT TTGTCTGACT AGGTGTCCTC TATAATATTA TGGGGTGGAG GGGGGTGGTA
TGGAGCAAGG GGCCCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTCGGGAA
CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA
GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC
AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC
AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG
TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC
GTCCAGACAC AGCATAGGCT ACCTGCCATG GCCCAACCGG TGGGACATTT GAGTTGCTTG
CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG
CGGCCAGCTT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC
AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAAGTCCC
CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG
TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC
CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC
TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCTCG
AGGAACTGAA AAACCAGAAA GTTAATTCCC TATAGTGAGT CGTATTAAAT TCGTAATCAT
GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG
CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC
TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG
GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA
TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT
CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG
GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC
TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC
AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC
GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC
GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC
CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA
GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT
GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA
GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA
TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG
GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC
AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC
AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA
TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA
GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC
CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT
TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC
CGGCTTTCCC CGTCAAGCTC TAAATCGGGG CATCCCTTTA GGGTTCCGAT TTAGTGCTTT
ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC
CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT
GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT
TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA
TTTTAACAAA ATATTAAACG TTTACAATTT
//
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