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Ron Lab
Lab protocols
Heather Harding's protocol for detection of PERK or GCN2 protein in cell lysates or tissue extracts by combined immunoprecipitation western immunobloting (download pdf)
Heather Harding's protocol for detection of phosphorylated eIF2a by western blotting (download pdf)
Heather Harding & Huiqing Zeng's protocol for isolating nuclear and cytoplasmic extracts
Heather Harding's protocol for ES cell culture
Choosing template DNA to construct a targeting vector for homologous recombination in ES cells
Culturing cells with defective ISR
Anne Bertolotti's protocol for culture of AR42J cells (download pdf)
Heather Harding's protocol for immortalizing MEFs with SV-40 large T antigen (download pdf)
Marcie Calfon's & Heather Harding's protocol for detection of in vivo DCF fluorescence in C. elegans
Marcie
Calfon's & Heather Harding's current protocols for detection of XBP-1 processing
in mouse or human cells
Heather Harding's protocol for cleaning worms with bleach, includes Arza Ron's procedure for neutralizing the bleach with thiosulfate
Cristina Benedetti's protocol for genotyping dead eggs of C. elegans
How to find 129sv BACs for your gene
How to obtain
full-length cDNA clones
Protocols related to mutant mice
How to obtain mice from the Ron Lab collection
Detection of AIRAP genotype in knockout mice
Detection of Alb::A1 (A.K.A. Alb::GADD34(+) transgene
Detection of
ATF4 genotype (Leiden's mutation) and wildtype
Detection of CHOP genotype in knockout mice
Detection of CRE in transgenic mice
Detection of GADD34 genotype in knockout mice (WT & KO1 Ex allele)
Detection of GCN2 genotype in KO4 knockout mice and cells
Detection of IRE1a genotype in knockout mice and cell lines
Detection of IRE1b genotype in knockout mice
Detection of P58ipk/DnajC3 genotype in knockout mice
Detection of PERK genotype in knockout mice and cell lines
Mouse numbering system in use by our lab (view gif)
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