Detection of Alb-haA1 transgenic mouse allele by genomic DNA PCR analysis
DNA prep:
	Prepare genomic DNA using proteinaseK method. (the NaOH method is less effective)
PCR primer
	Sense	haGADD34.1S	5’ CGG GCT GCA GGA ATT CGA TCT CGT T 3’
	Antisense	haGADD34. 6AS	5’ CTG TGT CCT CTC CAG GGC AAC AGA C 3’
PCR fragment
			335	bp	only in transgenic mouse
PCR solution
		set	×1
		ddH2O	18.05
		10× buf	2.5
		2.5 mM dNTP	1.25
		20 uM SP	0.5
		20 uM AP	0.5
		homemade Taq	0.2
		genomic DNA *	2
			25
		homemade 10x buffer (500mM Tris pH 9.0, 200mM NH4SO4, 15mM MgCl2)
	* DNA dilution (1/20-1/50) may increase sensitivity of PCR reaction
PCR protocol
		Denature	96 ℃	5 min
		Denature	94 ℃	1 min	35 – 40 cycles
		Anneal	55 ℃	1 min
		Extend	72 ℃	1 min
		Extend	72 ℃	7 min
			End