Cre  PCR

10xbuffer

100mM Tris-HCl pH8.3

500mM KCl

 15mM MgCl2  

Reaction

10x buffer *                             2.5ml

2.5mMdNTPmix                   2.5 ml

Cre1S(10pmol/ ml)   1 ml (GGG ATT GCT TAT AAC ACC CTG TTA CG)

Cre2AS(10pmol/ ml)   1 ml (TAT TCG GAT CAT CAG CTA CAC CAG AG)

H2O  16 ml

Tail  DNA  2 ml

Condition:   94C 3min-(94C 1mim- 60C 1min-72C 1min 30 cycle)-72 10min

PCR product: 213 bp

David Ron copied January 26, 2004

CRE PCR

(based on Masahiko’s 1997 protocol)

Primers were ordered May 1997