Published article:
He, W., P. Cowin, D.L. Stokes. 2003. Untangling Desmosomal Knots with Electron Tomography. Science 302: 109-113.

This supplementary material can also be found on the web site of Science Magazine and contains:

Materials and Methods
SOM Text
Figs. S1 to S5
Tables S1 and S2
References

In addition, three movies are available as part of these supplementary materials:

Movie S1 (mpeg - 1.5 Mbytes)

Scanning through the sections composing the tomographic map of desmosome "R". This same map was used for Fig. 1e,f. These sections have been cut parallel to the untilted sample, same as Fig. 1e and the movie therefore starts and ends with the upper and lower faces of the section. Densities have been segmented with colored lines both in the intercellular space and in the cytoplasmic plaque; colors used are blue for cadherins, red for the membrane, orange and light green for the two zones composing the cytoplasmic plaque, and dark green for the intermediate filaments. Discrete densities are clearly seen crossing the intercellular space that correspond to cadherin molecules. The large black circles at one end of the section correspond to the colloidal gold particles that were deposited on one surface of the section.

Movie S2 (mpeg - 3 Mbytes)

Scanning through the sections composing the tomographic map of desmosome "P". This map was used for extensive molecular delineation and fitting depicted in Fig. 2. These sections have been cut parallel to the untilted sample as in Fig. 1e and in video 1. Segmentation is shown in the forward half of the movie for the membrane (cyan) and a significant number of individual cadherin molecules (various colors). The colloidal gold particles are visible at one surface of the section and their larger size reflects the higher magnification used for desmosome "P" relative to "R" (see Table 1).

Movie S3 (mpeg - 8 Mbytes)

This video illustrates the use of Amira to compare segmented densities as well as fitted coordinates with the original densities in the original tomogram. Sections through the tomogram appear as black and white and are scanned backward and forward to intersect with the 3D shapes of segmented densities and fitted coordinates. Although the movie starts with the majority of desmosome P, it focuses on several molecules toward one end of the desmosome, which includes some of the same molecules shown in Fig. 2b. As expected, high densities are present in the map wherever the section intersects the 3D shapes. This is shown for both X-Y and X-Z sections.